目的：本文以糖原合成酶激酶3β（glycogen synthase kinase 3 beta，Gsk3β）为研究对象，探讨Gsk3β基因在红鲫胚胎发育及成体组织中的表达模式，以及在镉金属胁迫下的分子应答初探。方法：采用RACE克隆技术获得红鲫Gsk3β cDNA全长，半定量RTPCR技术和Western Blot技术分析Gsk3β在mRNA水平和蛋白水平的表达情况，最后通过特定浓度的镉处理分析Gsk3β的表达情况。结果：通过克隆发现Gsk3β基因全长为2 195 bp，5′端非编码区（UTR）共692 bp，3′非编码区有237 bp。编码含421个氨基酸的蛋白质，蛋白分子量约为46 846.40，等电点为9.05，且与斑马鱼和青鳉的亲缘关系最近。Gsk3β基因在胚胎发育和成体组织中呈现不同程度的表达，在不同组织中，Gsk3β在大脑组织表达最丰富，其次在眼睛，肌肉和精巢中表达相对较高，在卵巢、肝脏、肾脏和皮肤组织表达量相对较低。在不同发育时期，Gsk3β在两细胞期没有表达，之后在后续的不同时期呈现差异表达，出膜期表达最强，其次为肌效期、体节期、神经胚、囊胚期表达次之，在黑色素期和体色素期蛋白表达较弱。在镉处理后的红鲫肝脏中检测Gsk3β基因的表达，发现Gsk3β表达下调。结论：Gsk3β基因在红鲫胚胎发育中起着重要作用，且参与了镉金属胁迫应答，但其具体的分子机制仍有待进一步研究。

To investigate the expression pattern of Gsk3β gene in different developmental stages and adult tissues of red crucian carp, as well as its molecular response under cadmium stress. 〖WTHZ〗Methods:〖WTBZ〗The full length of Gsk3β cDNA was obtained by RACE cloning technique, which Gsk3β mRNA and protein was analyzed by semiquantitative RTPCR and Western Blot.The expression of Gsk3β was analyzed by specific concentration of cadmium. 〖WTHZ〗Results: 〖WTBZ〗The total length of Gsk3β gene was 2 195 bp,the 5′untranslated region (UTR) was 692 bp, and the 3′ untranslated region was 237 bp. Encoding 421 amino acids containing protein and protein molecular weight was 46 846.40, its isoelectric point was 9.05,and has the closest relationship with zebrafish and medaka.Gsk3β gene was expressed in different embryonic developmental stages and adult tissues.The expression of Gsk3β was most abundant in the brain tissue, and the expression of Gsk3β in the muscle and testis was higher than in the liver and kidney, ovaries and skin tissues. At different developmental stages, Gsk3β was not expressed in the twocell stage, and then expressed at different stages of followup. The expression of Gsk3β was the strongest in the last stage, followed by the duration of muscle, In the eye pigment period and somatic stage protein expression is weak. The expression of Gsk3β related genes were detected in the liver of red crucian carp after cadmium treatment, and the result showed that expression level of Gsk3β was in downregulation. 〖WTHZ〗Conclusion: 〖WTBZ〗Gsk3β gene plays an important role in different embryonic developmental stages of red crucian carp and participates in cadmium stress response, but its specific molecular mechanism remains to be further studied.