本研究通过NCBI数据分析得知Photorhabdus luminescens TT01基因组中表达Tc毒素蛋白复合体的基因簇包含了6个相关的Tc毒素基因，分别为tcaZCB（plu05140515）、tccAB3（plu08050806）、tccAB1（plu41654169）、tcd（plu09600971）、tccC4（plu0976）和tccC7（plu4488）。利用Red/ET直接克隆技术，快速获得发光杆菌TT01基因组DNA上的4个Tc毒素基因tcaZCB（6 804 bp）、tccAB3（7 419 bp）、tccAB1（10 954 bp）和tcd（51 406 bp）分别构建到相应的重组质粒p15AcmtcaZCB、p15AcmtccAB3、p15AcmtccAB1和p15Acmtcd上。通过PCR技术分别扩增得到tccC4基因（2 891 bp）和tccC7基因（2 871 bp），并进行T载体连接后，分别获得质粒pMD18tcc4和pMD18tcc7。本研究通过两种不同的生物技术方法成功克隆到发光杆菌TT01基因组上的Tc毒素蛋白复合体的所有相关基因，为后续的研究打下一定的基础。

In this research, genome sequencing data of Photorhabdus luminescens TT01 strains exhibited six secret genes related to the expression of the toxin complexes (Tc), including tcaZCB (plu05140515), tccAB3 (plu08050806), tccAB1 (plu41654169), tcd (plu09600971), tccC4 (plu0976) and tccC7 (plu4488), respectively. Firstly, four larger genes in TT01 chromosome named tcaZCB (6 804 bp), tccAB3 (7 419 bp), tccAB1 (10 954 bp) and tcd (51 406 bp) were rapidly fished out and constructed into vectors p15AcmtcaZCB, p15AcmtccAB3, p15Acm tccAB1, p15Acmtcd via direct cloning method. Then another two genes, tccC4 (2 891 bp) and tccC7 (2 871 bp) were gained by PCR amplification technique and formed recombinant vectors pMD18tcc4 and pMD18tcc7 by ligation with Tvector. Finally, using two different biotechnology methods, six episomal Tc genes in TT01 chromosome were successfully cloned. which would provide a positive foundation for the further study of different Tc toxins.