本文通过癌症靶向性分子叶酸（FA），硅壳包裹的TiO2（TS）纳米颗粒和5氨基乙酰丙酸（5ALA）进行结合形成新型光敏剂5ALATSFA，并研究其在光动力疗法（photodynamic therapy，PDT）治疗白血病肿瘤细胞HL60中的灭活效果。实验采用表面修饰的方法成功制备了5ALATSFA纳米颗粒，表征样品通过透射电镜（TEM）、傅里叶红外光谱（FTIR）、紫外可见光吸收光谱（UVVis）等测试方法，通过加入不同浓度的复合纳米颗粒5ALATSFA与HL60细胞共育12 h，分别测量纳米颗粒的暗毒性和PDT灭活效应，其中PDT灭活效应采用Cell Counting Kit8（CCK8）法检测。实验结果表明，5ALATSFA复合纳米颗粒分散性良好；当药物浓度为100 μg/mL与HL60细胞共育12 h后，5ALATSFA对HL60细胞的暗毒性较低，在光动力疗法中5ALATSFA灭活效率可达到74.65%，显著高于TiO2和TSFA的灭活效率。因此5ALATSFA作为一种潜在的光敏剂具备良好的应用前景。

A new photosensitizer 5ALATSFA was synthesized through the combination of cancer targeting molecule folate (FA), silica coated TiO2 (TS) nanoparticles and 5aminolevulinic acid (5ALA). Photodynamic therapy efficiency of 5ALATSFA on leukemia HL60 cell was studied in this paper. The composite nanoparticles (5ALATSFA) was synthesized by surface modification method and was characterized by transmission electron microscope (TEM), fourier transformation infrared spectrum (FTIR), ultravioletvisible absorption (UVVis) and so on. HL60 cells were cultured with different concentrations of composite nanoparticles 5ALATSFA for 12 h. The dark toxicity and PDT inactivation effect of nanoparticles were detected by Cell Counting Kit8 (CCK8) method. The experimental results show that the dispersibility of 5ALATSFA composite nanoparticles is good. When 5ALATSFA is incubated with HL60 cells for 12 hours at a concentration of 100 μg/mL, the dark toxicity of 5ALATSFA to HL60 cells is low and the 5ALATSFA inactivation efficiency of photodynamic therapy can reach 74.65%, which is significantly higher than that of TiO2 and TSFA. Therefore, 5ALATSFA has a good prospect of application as a potential photosensitizer.