本文以华中农业大学园艺植物教育部重点实验室细胞生物学实验平台现有的一套由德国徕卡公司生产的新型号TCSSP8激光共聚焦扫描显微镜机组（文中简称SP8）为例，结合实际操作经验，从机组组成使用、软件的使用、使用中常见问题、用后维护及保养等方面进行了大量总结和探索，希望能够为初次使用仪器者及致力于该领域的科研工作者提供第一手直观详实易懂的操作规程。

In order to provide reference for the researchers and technologies on how to use the Laser scanning confocal microscope efficiently, taking a set of Laser confocal laser scanning microscope (the model is TCSSP8) which was produced by Leica corporation in Germany as an example, a lot of suggestions and introdutctions about function, principle, structure, use of sofeware, instrumental management, experimental technology and  analysis of common problems in use were disserted in this paper