以筛选得到的四倍体稗草成熟胚作为外植体，进行了组织培养与快速繁殖技术的研究。结果表明：最佳外植体消毒方式为70%乙醇浸泡30 s+0.1%（w/v）的升汞消毒15 min。最佳诱导培养基为MS盐、DL维生素+2.5 mg/L 2,4-D+300 mg/L谷氨酰胺+500 mg/L脯氨酸+3%麦芽糖。将愈伤组织转入分化培养基（MS+50 mg/L肌醇+1.0 mg/L 6-BA+0.2 mg/L KT+0.5 mg/L NAA+0.25 mg/L IAA+3%麦芽糖）上，培养20 d后超过70%的愈伤组织分化成苗。将35 cm长的分化苗转入1/2 MS生根培养基进行生根培养。炼苗后，移入营养土与珍珠岩（2〖DK1〗∶1）的基质中，移栽成活率高达90%以上。该体系的建立为稗草抗除草剂基因的功能验证提供了前提条件。

We report a successful and efficient plant regeneration system using mature embryo of barnyardgrass (Echinochloa crus). The effect of different sterilization procedures on explant culture and the effect of plant growth regulators on regeneration process and acclimatization of transplanting were assessed. It was found that EtOH+0.1%HgCl2 was optimal for explant sterilization. Essentially, dehulled and sterilized seeds were plated on callus induction and subculture medium composed of MS basal salts and DL vitamins plus 2.5 mg/L 2,4D, 300 mg/L glutamine, 〖JP1〗500 mg/L proline, 3% maltose, 0.8% (w/v) agar at pH 5.8. Differentiation was carried out on a differentiation medium MS with 50 mg/L〖JP+1〗 inositol plus 1.0 mg/L 6BA, 0.2 mg/L kinetin KT, 0.5 mg/L NAA, 0.25 mg/L IAA, 3% maltose, 0.8% (w/v) agar at pH 5.8. Plantlets ranging from 3 to 5 cm in length were transferred to the rooting media comprising 1/2MS basal salts. In vitro regenerated plantlets were washed to remove the traces of agar and then were transferred to pots containing nutritious soil and perlite (2〖DK1〗∶1, v/v), and the survival rate was over 90%. The protocol developed in this study will facilitate barnyardgrass generation and is useful for the production of herbicideresistant transgenic plants.