为筛选生物钟核心基因per1表达定量中的相对稳定性最好的内参基因，本研究取翘嘴鳜成鱼心脏、肝脏、肾脏、脑、红肌、白肌、肠、眼和脾等九个组织为研究对象，选取GAPDH、18S rRNA、βactin、rps29、RPL13a、B2M和EF1a为内参基因，采用实时荧光定量PCR(qRTPCR)对per1基因mRNA表达水平进行检测分析。研究结果表明18S rRNA和GAPDH的平均稳定值M最低，相对表达量最稳定。以18S rRNA和GAPDH为内参基因时分析发现per1基因表达量在肝脏中最高。本研究为在鱼类per1 mRNA表达检测过程中选用稳定的内参基因提供了实验和理论参考。

For the screening the best reference gene of the biological clock core gene per1 expression in quantitative analysis, we compared the stability of the 7 reference genes (GAPDH, 18S rRNA, βactin, rps29, RPL13a, B2M and EF1a) in heart, brain, kidney, liver, slow muscle, fast muscle, intestine, spleen and eye tissues of the adult Siniperca chuatsi. By using GeNorm software, the results showed that the GAPDH and 18S rRNA are the the most stable reference genes in 9 tissues. With 18S rRNA and GAPDH as reference gene analysis of the expression of per1 in the liver was the highest. This study for the detection of per1 mRNA levels in fish tissues and in the selection of one or more reference genes provide experimental and theoretical reference.