#br# Comparison of the Transfection Efficiency through Liposome between #br# 16HBE and the Malignant Transformation Cells Induced by Nickel #br# Sulfide and AntiBenzo (a) pyrene
Abstract：Objective: To investigate the carcinogenesis of malignant transformation cells by comparing the difference in transfection efficiency of exogenous plasmids through liposome between normal cells and malignant transfected cells. Methods: Two types of plasmids that express the fluorescent proteins were transfected with liposome into cells such as 16HBE and its malignant transformation cells induced by crystalline nickel sulfide and antibenzo (a) pyrene separately, and the transfection were observed by fluorescent microscope. Results:In a range of cell density, the transfection efficiency of normal cells 16HBE is lower than that of the malignant transfected cells both with the plasmids eGFP and GFPLC3. Conclusion:Inconsistency in membrane permeability between normal cells and malignant transfected cells leads to inconsistency in transfection efficiency.
引用本文:
夏盛源，王远航，朱蔚云，张〓岚. 人支气管上皮细胞16HBE和其经硫化镍、反式BPDE诱导的恶性转化细胞对脂质体转染效率的差异性比较[J]. 激光生物学报, 2018, 27(2): 148-154.
XIA Shengyuan, WANG Yuanhang, ZHU Weiyun, ZHANG Lan. #br# Comparison of the Transfection Efficiency through Liposome between #br# 16HBE and the Malignant Transformation Cells Induced by Nickel #br# Sulfide and AntiBenzo (a) pyrene. journal1, 2018, 27(2): 148-154.
