Abstract:Human myeloid differentiation factor 88 (MyD88) is the central factor for inflammatory signaling pathways downstream of members of the Tolllike receptor (TLR) and interleukin1 (IL1) receptor families. To explore the human MyD88 structures and functions by bioinformatics analysis, results showed human MyD88 gene of full length 5 670 bp, located on chromosome 3p22.2, and encoded 296 amino acids, which was a higher conserved one. Meanwhile, human MyD88 was highly distributed in the blood, spleen and lung, with no signal peptides, whereas it included 2 sumolation sites and 15 serine phosphorylation sites, 6 threonine phosphorylation sites and 4 tyrosine phosphorylation sites. The secondary structure was mainly consisted of 145 αhelix and 30 βsheet regions, indicating that the tertiary structure was reliable by ramachandran plot. In addition, human MyD88 also has protein interaction with Tolllike receptors and interleukins, participating in inflammatory reaction, cell apoptosis and other immunologic processes. All together, our bioinformatics analysis would provide a basis for understanding structure features and biological functions of human MyD88, guiding a new schema for the diagnosis and treatment in the future.