Abstract：For the screening the best reference gene of the biological clock core gene per1 expression in quantitative analysis, we compared the stability of the 7 reference genes (GAPDH, 18S rRNA, βactin, rps29, RPL13a, B2M and EF1a) in heart, brain, kidney, liver, slow muscle, fast muscle, intestine, spleen and eye tissues of the adult Siniperca chuatsi. By using GeNorm software, the results showed that the GAPDH and 18S rRNA are the the most stable reference genes in 9 tissues. With 18S rRNA and GAPDH as reference gene analysis of the expression of per1 in the liver was the highest. This study for the detection of per1 mRNA levels in fish tissues and in the selection of one or more reference genes provide experimental and theoretical reference.
