Abstract：Objective: To investigate the possible mechanism of effects of Burn Ointment on burn wound healing of the skin tissue in scalded model mice. Methods：Fifty mice were divided into 5 groups randomly: control group, model group, high dose group, middle dose group and low dose group of Burn Ointment. The experimental groups were applied by Burn Ointment for 15 days except the control group and model group. After treatment, skin thickness and cell pathomorphology were detected by Masson staining. Moreover, content of hydroxyproline (Hyp) was measured by biochemical method. Contents of TNFα，IL1β, Collagen I,EGF and TGFβ1 were detected by ELISA. Results：Compared with control group, from the observation of the scalded wound surface of mice, we found that structure of skin was unclear and epidermal cells were detached. Cells appeared degeneration and necrosis. The thickness of the epidermis and dermis was reduced. Collagen fibers were thinner, disordered and fractured. The contents of Hyp, TNF and IL1β of the model group were all significantly higher than those of control group (P all<0.01), while the contents of EGF, TGFβ1 and Collagen I were decreased markedly (P all<0.01). After Burn Ointment treatment, every indicator of experiment was significantly improved. Conclusion：The Burn Ointment can significantly inhibit early wound infection, reduce breakdown of collagen and promote wound healing, which possibly related with inhibition of inflammatory cytokines and promotion of growth factors.
