Abstract:The aim of this study was to investigate the effects of dietary supplementation with chinese chives for Hyline Brown breeder roosters on the hatching of breeding eggs in Hyline Brown Layers. Four hundred of 4weeksold Hyline Brown breeder roosters were randomly assigned to four groups equally (n=20). Each treatment was replicated five times. Control group Ⅰwas fed with basal diet and treatment groups were fed with the basal diet supplemented with 1%, 3% and 5% chinese chives (test Ⅱ, Ⅲ, Ⅳ) to Hyline Brown breeder roosters. The artificial insemination was done after 16 weeks. The A, B, C and D groups in 24weeksold Hyline Brown laying breeders were artificially inseminated by Ⅰ, Ⅱ, Ⅲ and Ⅳ groups of semen of Hyline Brown breeder roosters, one rooster to 20 laying breeders, respectively. Five hundred breeding eggs were randomly selected to incubate after the 4th day of insemination. The results showed that fertility, hatchability, thrifty chick rate and average birth weight were significantly increased by chinese chives supplementation at level 1%, 3% and 5% (P<0.05). In addition, the usage of 5% chives suggested that it would be preferably added to Hyline Brown breeder roosters’ diets. It could be concluded from this study that chinese chives can be a promising dietary supplement to increase breeding eggs hatching in the poultry industry.