Abstract:In order to investigate the molecular biological mechanism involved in germination of soybean young embryos promoted by high temperature(HT), both young embryos of soybean cultivar “Nipponbare” treated with HT and without HT were employed to screen the differential expression genes with mRNA differential display reverse transcriptionpolymerase chain reaction (DDRTPCR). In this study, we reported K6, one of the differential expression genes. K6 was regarded as homologue of Lea5 gene (soybean cultivar Williams 82), because they shared 99% identical nucleotides. Blastp analysis showes that the putative protein of K6 contains LEA type3 motifs and is assigned to LEA3 superfamily. Analysis by CLC Protein workbench 5 software also showes that the putative protein has 113 amino acid residues with relative MW 12.283 kD and pI 10.12. The putative protein of K6 has no typical secondary structure element. Results of RTPCR suggested that expression of K6 gene is constitutive. Expression levels of the gene in soybean young embryos developmental process was relatively constant. Moreover, expressions of the gene were also detected in roots, hypocotyls and leaves.