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Quick Search     Advanced Search   2024 Vol.  33 No.  3 Published: 2024-06-28 1 2024 Vol. 33 (3): 1-2 [Abstract] ( 156 ) [HTML 1KB] [PDF 795KB] ( 1119 ) 193 LI Xiaochun, SONG Kai, CHEN Bo, JIANG Lian, HE Yawen Plant Growth-promoting Rhizobacteria: Mechanisms and Perspectives (1. State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China; 2. Jiangsu Good Harvest-Weien Agrochemical Co. Ltd., Qidong 226200, China) Abstract: The bacteria that live in the rhizosphere and promote the growth of host plants are called plant growth-promoting rhizobacteria (PGPR). PGPR application helps to reduce the use of pesticides and fertilizers and contributes to the sustainable development of agriculture. Over the past 20 years, significant progresses have been made in both theoretical and applied researches of PGPR. This review aims to summarize the mechanisms underlying PGPR growth promotion and disease resistance, including improving nutrient supply and uptake, producing phytohormone and signal molecules, synthesizing volatile organic compounds, and secreting antibiotics. The future research direction of PGPR in both basic and application aspects is also discussed, hoping to provide theoretical reference for future PGPR related research. Key words: plant growth-promoting rhizobacteria; phytohormone; signaling molecule; microbial volatile organic compounds （Acta Laser Biology Sinica, 2024, 33(3): 193-200） 2024 Vol. 33 (3): 193-202 [Abstract] ( 194 ) [HTML 1KB] [PDF 2187KB] ( 1173 ) 201 ZENG Xin, CAI Xiaoyan, LI Fang Research on the Role of Folic Acid in the Development of Animal Embryonic Organs (Center of Heart Development，College of Life Science，Hunan Normal University，Changsha 410081，China) Abstract: Folic acid, also known as vitamin B9, plays a key role in DNA synthesis, RNA transcription, homocysteine to methionine and cellular metabolism. Embryonic development is regulated by both environmental and genetic factors. Folic acid is an important nutrient for the normal development of animal embryonic organs. Maternal folic acid supplementation can reduce the risk of congenital organ dysplasia, such as heart, eyes, neural tube and kidney development abnormalities. In this study, we elucidates the involvement of folic acid transport, folic acid metabolism and the relationship between folic acid supplementation and epigenetic modifications in early embryonic organ development. In addition, we analyzed the effect of folic acid deficiency on the development of animal embryonic organs and the importance of folic acid supplementation during pregnancy, providing a theoretical basis for the prevention and treatment of abnormal embryonic organ development in the future. Key words: folate acid; embryonic organ development; folate acid metabolism; DNA methylation; epigenetic modifications （Acta Laser Biology Sinica, 2024, 33(3): 201-208） 2024 Vol. 33 (3): 201-208 [Abstract] ( 177 ) [HTML 1KB] [PDF 1940KB] ( 1114 ) 209 ZHENG Wenhu, LI Hui, CHEN Chong, WANG Linbo Segmentation and Quantification of Zebrafish Hindbrain Vessel Confocal Images Based on DE-3D U-Net (1. School of Biomedical Engineering, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230026, China; 2. Jiangsu Key Laboratory of Medical Optics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China) Abstract: The choroid plexues in the zebrafish hindbrain controls the exchange of substances between brain and its vessels, and the vascular abnormalities around the choroid plexues can lead to associated cerebrovascular diseases. We first performed serial imaging of the major blood vessels in the hindbrain region of zebrafish, and obtained a series of images of zebrafish between 43 hours and 63 hours after fertilization using confocal microscopy to photograph embryos under normal conditions and after cylindrospermopsin treatment. Afterwards, the DE-3D U-Net was developed for the segmentation of the hindbrain images. The segmentation results showed that the class pixel accuracy of DE-3D U-Net could reach to 86.67%, 93.18% and 83.74% for the three main vessels and the segmentation results were used to plot vascularization curves. The quantitative results showed that the radii of the three vessels showed a reduction of 18% after treatment with high concentrations of cylindrospermopsin, and the angle of the mesencephalic vein showed a reduction of 30° to 50°. This study proposed a neural network for segmentation of hindbrain vessels of zebrafish, and it provided a basis for research on the development of zebrafish choroid plexsus. Key words: zebrafish; hindbrain vessels; confocal microscopy; 3D U-Net deep-learning network; cylindrospermopsin （Acta Laser Biology Sinica, 2024, 33(3): 209-216） 2024 Vol. 33 (3): 209-216 [Abstract] ( 187 ) [HTML 1KB] [PDF 3217KB] ( 1180 ) 217 GUAN Hua, ZHANG Jian, RUAN Delin, ZHANG Xinxin, ZHANG Tao, YANG Liping, ZHANG Ningjie, YAN Liang, JIA Erhui Optimization Design of Sequencing Module for Domestic Genetic Analyzer#br# (1. First Research Institute of the Ministry of Public Security of PRC, Beijing 100048, China; 2. Institute of Forensic Science of China, Beijing 100038, China; 3. Bio-evidence Sciences Academy, Xi’an Jiaotong University & National Biosafety Evidence Foundation (NBEF), Xi’an 710049, China; 4. Beijing Zhongdun Anmin Analysis Technology CO. Ltd., Beijing 102200, China) Abstract: A domestic 24 channels genetic analyzer combined with a domestic 36 cm capillary arrays were used to solve the problems of fluorescence signal correction, running voltage, and migration correction during sequencing on the genetic analyzer based on non-gel sieving capillary electrophoresis technology. Spectral calibration model and appropriate baseline noise values were obtained under the condition that the original spectral fluorescence signal of the analyzer was used as a regulation basis. A standard curve was plotted by combining an running voltage and a base spacing value, while a clear range length was also calculated by a sequencing analysis software, so as to determine the optimal running voltage and base spacing. In addition, a migration offset value was further calculated and a linear model was eventually established between the base size and the migration time, which achieved an accurate identification of bases. It was reported that with the appropriate spectral calibration model and baseline noise threshold limitation, the strongest ability to detect the longest clear range length, that is 561 bp, was found in the analyzer when the running voltage was set to 10 kV and the base spacing was 13.05 frames. By compensating for migration corrections, a linear model was established between the base size and the migration time. Among them, R2 values of bases G, A, T, and C increased from 0.992 7, 0.992 7, 0.994 5, and 0.987 9 to 0.999 6, 0.999 8, 0.999 6, and 0.999 7, respectively. After correction, all fluorescent bases were accurately labeled without any omissions or errors, clear range length extended to 621 bp. This study could better guide the optimization and design of a sequencing module of the domestic genetic analyzer, making the DNA base recognition function of the analyzer more efficient and accurate. Key words: domestic genetic analyzer;  non-gel capillary electrophoresis; base  sequencing; run voltage;  migration correction （Acta Laser Biology Sinica, 2024, 33(3): 217-226） 2024 Vol. 33 (3): 217-226 [Abstract] ( 204 ) [HTML 1KB] [PDF 9016KB] ( 1059 ) 227 YANG Yue, WANG Hui, WANG Rui, MA Ke, XU Ying, ZHANG Shuijun, ZHANG Zhenzhen, CHEN Rong, MENG Qingguo, FENG Zhixin Expression, Purification, and Preliminary Crystallographic Study of Ferritin from Spiroplasma eriocheiris#br#  (1. School of Marine Science and Engineering, Nanjing Normal University, Nanjing 210023, China; 2. Key Laboratory of Veterinary Biological Products Engineering, Ministry of Agriculture and Rural Affairs, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; 3. School of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China; 4. Guotai Technology Innovation Center of Veterinary Biological Products (Taizhou), Taizhou 225300, China) Abstract: Ferritin is a class of proteins ubiquitously found in various types of nucleated organisms and involved in the iron homeostasis regulation of body. Ferritin can self-assemble to form a hollow nanocage of about 14 nm in size and is often used as carrier for drug delivery and vaccine construction. The crystal structure of Mycoplasma ferritin shows atypical ferroxidase centres and new pathway for Fe2+ uptake, which are structurally different from those of other species. Not much work has been reported for Spiroplasma ferritin. Here, our studies show that Spiroplasma ferritin has low sequence similarity with other ferritins including Mycoplasma ferritins. Spiroplasma eriocheiris Ferritin (SeFer) was recombinant expressed in E. coli in soluble form after IPTG (isopropyl-beta-D-thiogalactopyranoside) induction. High-purity SeFer protein was obtained after purification by DEAE (diethylaminoethyl) weak anion exchange chromatography and gel filtration. Crystal screen of SeFer was performed by sitting drop vapor diffusion at 4℃ and 18℃. SeFer crystals grew in several screen conditions. The best crystal diffracted to 2.90 Å by synchrotron radiation source and it belonged to the I432 space group. The X-ray crystallography data collected here can be used for further structure determination. Native-PAGE, dynamic light scattering, and the position of peaks of SeFer in molecular sieve showed that the molecular weight of SeFer is about 400.0 kD, and the diameter of SeFer was about 15 nm, both of them are close to the theoretical value. The structural model of SeFer was predicted by using Alphafold 2. The predicted model shows that SeFer has a regular icosahedral overall structure, which is similar to other ferritins. However, SeFer model shows that the key residues of ferroxidase center differ from those of mycoplasma ferritin. Our results not only provide a basis for studying the crystal structure of SeFer, but also provide new candidates for vaccine vectors. Key words: Spiroplasma eriocheiris; ferritin; protein purification; crystal structure （Acta Laser Biology Sinica, 2024, 33(3): 227-235） 2024 Vol. 33 (3): 227-235 [Abstract] ( 164 ) [HTML 1KB] [PDF 3498KB] ( 972 ) 236 YANG Jing, LIU Fen, LI Yi, WU Dongbo, DENG Mandan, YANG Chengying, YIN Chen, HU Xiang Mechanism Study of Human Umbilical Cord Mesenchymal Stem Cells in the Treatment of Mice with Gestational Diabetes#br# (1. Department of Obstetrics and Gynecology, the First Hospital of Changsha, Changsha 410005, China; 2. College of Life Sciences, Hunan Normal University, Changsha 410081, China) Abstracts: This study aimed to investigate the the effectiveness and mechanism of human umbilical cord mesenchymal stem cells (hUCMSCs) in mice with gestational diabetes mellitus (GDM) model, and to study a new method for the treatment of GDM. hUCMSCs were obtained by tissue block culture. Female mice were raised with intraperitoneal injection of streptozotocin (STZ) solution to induce type 2 diabetes after being fed with D12451 high-fat. Then, mice with type 2 diabetes mellitus were kept in cages overnight, both male and female. Sperm were examined the next morning using pubic plugs and microscopic sperm, which was recorded as the first day of gestation, and fasting blood glucose was measured on the fourth day of gestation. The animals with fasting blood glucose higher than of 11.2 mmol/L and polydipsia, polyphagia, and polyuria were designated as GDM model mice. After injecting 5×105 hUCMSCs into the tail vein to treat GDM model mice, the effects of hUCMSCs on GDM mice were analyzed by detecting changes in blood glucose levels，serum inflammatory factors, macrophages, pathological sections of islet cells, and other indicators. Blood glucose levels of GDM mice treated with hUCMSCs were significantly reduced (0.001<P<0.01). Immunohistochemical results showed that the ratio of islet-β-cells to α-cells increased significantly in the mice after treatment with hUCMSCs (0.001<P<0.01). The results of inflammatory factor detection showed that the expression of pro-inflammatory factor IL-1β was reduced in the treatment group compared with the control group(P<0.05). In vitro cell experiments showed an increase in the expression of M1 macrophages co-cultured with hUCMSCs. It indicated that hUCMSCs can reduce the blood glucose level of GDM mice to a certain extent and effectively affect their immunomodulatory effect，which is beneficial to the treatment of GDM in mice. Key words: human umbilical cord mesenchymal stem cells; gestational diabetes mellitus; inflammatory factors; sugar metabolism; model mice （Acta Laser Biology Sinica, 2024, 33(3): 236-242） 2024 Vol. 33 (3): 236-242 [Abstract] ( 181 ) [HTML 1KB] [PDF 2644KB] ( 1017 ) 243 FENG Ling, HUANG Zhaoxia, LI Junlan, DONG Zhengping, ZHANG Yongqin A Study on Mechanism of ASM in the Treatment of ALI Based on Network Pharmacology and Molecular Docking#br# (Guizhou University of Traditional Chinese Medicine a. Department of Basic Medicine; b. Department of Pharmacy, Guiyang 550025, China) Abstract: Based on network pharmacology and molecular docking technology, the mechanism of radix angelicae sinensis-radix salviae miltiorrhizae drug pair (ASM) in the treatment of acute liver injury (ALI) was studied and verified by experiment. In this study, network pharmacology and molecular docking methods were used to screen the core targets of ASM in the treatment of ALI, and the core targets were enriched by analysis, and protein interaction network was constructed. The core target was molecular docked with the active components of ASM, and the molecular docking was verified by constructing ALI rat model and detecting the expression of relevant serum biochemical indexes and liver proteins. The results showed that 67 active ingredients were obtained from ASM, of which 75 targets interacted with acute liver injury. Protein-protein interactions (PPI) network analysis showed that TP53, CASP3, JUN, STAT3, AKT1, VEGFA, TNF, IL-6, MMP9 and PTGS2 may be the key targets of ASM in the treatment of ALI. The core targets of GO and KEGG signaling pathway enrichment analysis were mainly concentrated in hepatitis B signaling pathway, lipid and atherosclerosis, AGE-RAGE, and apoptosis signaling pathways. Molecular docking results showed that the core targets (TP53 and CASP3) had good binding sites with β-sitosterol, baicalin and cryptotanshinone. The results of in vivo experiments showed that ASM could reduce the expression levels of ALT, AST and MDA in serum of CCl4-induced model rats, and increase the activity of SOD, CAT and GSH in liver, thereby alleviating the CCl4-induced oxidative stress. The ASM could up-regulate BCL2 and down-regulate the expression levels of BAX, CASP3 and TP53. The results of this study indicate that ASM can effectively ameliorate ALI, which may be achieved through β-sitosterol, baicalin and cryptotanshinone and other chemical components, acting on key targets such as TP53 and CASP3, and through multiple signaling pathways such as p53 signaling pathway and apoptosis pathway. This study provides theoretical and experimental basis for clinical ASM treatment of ALI, and provides new ideas and methods for further research and development of traditional Chinese medicine. Key words: radix angelicae sinensis; radix salviae miltiorrhizae; acute liver injury; network pharmacology; molecular docking （Acta Laser Biology Sinica, 2024, 33(3): 243-252） 2024 Vol. 33 (3): 243-252 [Abstract] ( 181 ) [HTML 1KB] [PDF 5331KB] ( 970 ) 253 LU Xun, LU Jianhua, PENG Shuming, XIA Qingzhu, PENG Bo, LI Benben, ZHANG Xiaobo Evolutional and Functional Analysis of Plant GYF Domains (1. Agricultural Science Academy of Xiangxi Tujia and Miao Autonomous Prefecture, Xiangxi 416000, China; 2. College of Life and Health Science, Kaili University, Kaili 556011, China; 3. Pingjiang County Agriculture and Rural Bureau Plant Protection and Inspection Station, Yueyang 414500, China） Abstract: According to the extensive distribution of GYF domain proteins and the diversity of its functions, it is necessary to study GYF domain proteins further and conduct evolutionary analysis. Since AtEXA1 is the most well-studied GYF domain protein in plants, this study mainly focuses on the proteins similar to AtEXA1. Analysis of 8 053 GYF domain proteins in eukaryotes ranging from unicellular lower fungi to higher organisms showed that AtEXA1-like proteins are mainly found in plant species, while GYF proteins from animals have low similarity to AtEXA1. It was also found that AtEXA1-like proteins are present in almost all agricultural crops. This study demonstrates the conservation of all GYF domain proteins, initially deconstructs the evolution of GYF domain proteins, and provides implications for subsequent studies on the functional characteristics of GYF domain proteins. Key words: GYF domain; conserved domain; protein evolution; protein function （Acta Laser Biology Sinica, 2024, 33(3): 253-258） 2024 Vol. 33 (3): 253-258 [Abstract] ( 175 ) [HTML 1KB] [PDF 3992KB] ( 1156 ) 259 XING Jianhua, SUN Lulu, LI Zhexian Catechin Alleviates Apoptosis, Inflammation and Oxidative Damage of Rat Cardiomyocytes Induced by Lipopolysaccharide by Inhibiting p38 MAPK Phosphorylation (Department Geriatric Medicine, Hebei China Petroleum Central Hospital, Langfang 065000, China) Abstract: To investigate the effects of catechins on oxidative damage, inflammation and apoptosis of rat cardiomyocytes (H9C2) induced by lipopolysaccharide (LPS), as well as the regulation of p38 mitogen-activated protein kinase (MAPK) signaling pathway, H9C2 cells were cultured in vitro and divided into the control group which is without intervention, LPS group with 10 μg/mL LPS treatment, catechin with different concentrations (20, 40, 80, 160 nmol/L catechin treatment based on LPS group) and SB203580 group (10 μg/mL LPS+1 μmol/L SB203580 treatment), inhibitor group (10 μg/mL LPS+160 nmol/L catechin +1 μmol/L p38 MAPK pathway inhibitor SB203580 treatment) and activator group (10 μg/mL LPS+160 nmol/L catechin +10 μmol/L p38 MAPK pathway activator C16-PAF). Cell viability was measured with cell counting kit-8 (CCK-8) after 24 h treatment. The levels of interleukin-6 (IL-6) and interleukin-10 (IL-10) were detected by enzyme-linked immunosorbent assay (ELISA). Malondialdehyde (MDA) and superoxide dismutase (SOD) kits were used to detect the expression levels of oxidative stress factors MDA and SOD in cell supernatant. The apoptosis rate was determined by Hoechst 33258 staining. The expression levels of Caspase-3 and p38 MAPK pathway-related proteins were determined by Western blot. Compared with the control group, the cell viability of LPS group significantly decreased (P<0.05). Compared with LPS group, the cell viability significantly increased after 160 nmol/L catechin supplementation (P<0.05). Finally, 160 nmol/L catechin group was selected as catechin group for follow-up experiment. In the follow-up experiment, compared with the control group, SOD and IL-10 contents in LPS group significantly decreased (P<0.05), MDA and IL-6 contents, apoptosis number, Caspase-3 and P-P38 MAPK protein expression significantly increased (P<0.05); compared with LPS group, catechin group and SB203580 group significantly reversed the changes of the above indexes (P<0.05); compared with the catechin group, SB203580 in the inhibitor group enhanced the changes in the above indicators, while C16-PAF in the activator group attenuated the effect of catechin on LPS-induced H9C2 cells (P<0.05). In this study, catechins can significantly inhibit the apoptosis, inflammation and oxidative stress injury of H9C2 cells induced by LPS, and the mechanism of action may be related to the inhibition of p38 MAPK pathway signal transduction. Key words: catechins; cardiomyocytes; lipopolysaccharide; p38 mitogen-activated protein kinase signaling pathway; oxidative damage （Acta Laser Biology Sinica, 2024, 33(3): 259-266） 2024 Vol. 33 (3): 259-266 [Abstract] ( 154 ) [HTML 1KB] [PDF 2739KB] ( 930 ) 267 WEN Ling, LI Baoqi, ZHAO Yanmin, ZHENG Shuyang, SU Ying Effect and Mechanism of Azithromycin on Inflammatory Injury of Alveolar Epithelial Cells Induced by Lipopolysaccharide (The First Hospital of Qinhuangdao a. Pediatric ICU; b. College of Pharmacy, Qinhuangdao 066000, China) Abstract: To explore the regulatory effects of azithromycin on lipopolysaccharide (LPS) -induced proliferation, apoptosis and Janus kinase 2 (JAK2)/signal transduction and transcription promoter 3 (STAT3) signaling pathways in human alveolar epithelial cells, in vitro culture of human alveolar epithelial cells A549, were divided into blank group (no intervention), LPS group (10 μg/mL LPS treated for 24 h), low/medium/high concentration experimental group (10 μg/mL LPS+1, 2, 4 μg/mL azithromycin) and azithromycin group (10 μg/mL LPS+4 μg/mL azithromycin), inhibitor group (10 μg/mL LPS+50 μmol/L JAK2/STAT3 pathway inhibitor AG490), azithromycin+inhibitor group (10 μg/mL LPS+4 μg/mL azithromycin+50 μmol/L AG490) and azithromycin+activator group (10 μg/mL LPS+4 μg/mL azithromycin+0.5 μmol/L JAK2/STAT3 pathway activator Colivelin). After 24 hours of intervention, enzyme-linked immunosorption assay (ELISA), cell counting kit-8 (CCK-8), 5-acetyney-2' deoxyuracil nucleoside (EdU), Hoechst 33258 staining and Western blotting (WB) were used for the expression levels of inflammatory factors interleukin-6 (IL-6), interleukin-8 (IL-8), tumor necrosis factor-α (TNF-α), cell viability, proliferation rate, apoptosis rate, Caspase-3 and Cyclin D1 and the expression levels of JAK2/STAT3 signaling pathway related proteins. The results show that: compared with blank group, the expression levels of inflammatory cytokines IL-6, IL-8 and TNF-α in LPS group significantly increased, and cell viability significantly decreased. Compared with LPS group, the expression levels of inflammatory cytokines IL-6, IL-8 and TNF-α in high-concentration experimental group significantly decreased, and cell viability significantly increased. In this study, 4 μg/mL azithromycin with significant difference from LPS group was selected as azithromycin group for follow-up experiments. Compared with blank group, the cell proliferation rate and Cyclin D1 protein expression levels in LPS group significantly decreased, while the apoptosis rate, Caspase-3, p-JAK2 and p-STAT3 protein expression levels significantly increased. Compared with LPS group, azithromycin group and inhibitor group significantly reversed the changes of the above indexes. Compared with azithromycin group, cell proliferation rate and Cyclin D1 protein expression levels in azithromycin+inhibitor group further significantly increased, while apoptosis rate, IL-6, IL-8, TNF-α, Caspase-3, p-JAK2 and p-STAT3 protein expression levels further significantly decreased. Azithromycin+activator group significantly reversed the changes of the above indexes. Azithromycin can reduce LPS-induced inflammatory damage of A549 cells by inhibiting JAK2/STAT3 signaling pathway, promote cell proliferation and inhibit apoptosis, and provide evidence for exploring azithromycin treatment of LPS induced acute lung injury. Key words: azithromycin; Janus kinase 2; signal transduction and transcription promoter 3; alveolar epithelial cells; inflammatory lung injury （Acta Laser Biology Sinica, 2024, 33(3): 267-274） 2024 Vol. 33 (3): 267-274 [Abstract] ( 163 ) [HTML 1KB] [PDF 3653KB] ( 1009 ) 275 LIU Danyang, WU Zhenyong, SUN Yaru, CUI Yuxiu Protective Effects of Metformin Mediated NF-κB Signaling Pathway on Cell Damage in Diabetic Nephropathy (Department of Laboratory Medicine, the Second Central Hospital of Baoding, Baoding 072750, China) Abstract: In order to explore the protective effect of metformin mediated nuclear factor-κB (NF-κB) signaling pathway on the damage of diabetic nephropathy cells, HGPC cells were cultured in vitro and treated with 10, 20, 30 and 40 mmol/L D-glucose to select the optimal concentration of D-glucose for the construction of HGPC hyperglycemic inflammatory injury model. Then 20, 40, 80 and 160 mmol/L metformin were used for intervention, and the optimal concentration of metformin was screened. The cells were then divided into control group (Con group), high glucose group (HG group) and metformin group (Met group, 30 mmol/L D-glucose+80 mmol/L metformin), inhibitor group (Y group, 30 mmol/L D-glucose+1 μmol/L NF-κB pathway inhibitor BAY 11-7082), metformin+inhibitor group (Met+Y group, 30 mmol/L D-glucose+80 mmol/L metformin+1 μmol/L BAY 11-7082) and metformin+agonist groups (Met+A group, 30 mmol/L D-glucose+80 mmol/L metformin+1 μmol/L NF-κB pathway agonist Prostratin), intervention was conducted for 24 h. Cell count kit 8 (CCK-8) was used to detect cell viability; the expression levels of inflammatory cytokines were detected by enzyme-linked immunosorbent assay (ELISA); cell invasion and migration were determined by Transwell assay. Western blotting (WB) was used to detect the expression levels of E-cadherin, N-cadherin, vimentin, Fibronectin (FN), NF-κB p65 and p-NF-κB p65. The results show that: HGPC cells were treated with 30 mmol/L D-glucose for 24 h to construct HGPC hyperglycemic inflammation model, and 80 mmol/L was the optimal concentration of metformin. Compared with Con group, the levels of inflammatory cytokines tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), invasion ability, migration ability, the expression levels of N-cadherin, vimentin, FN and p-NF-κB p65 protein in HG group significantly increased, while the expression level of E-cadherin protein decreased (P<0.05). Compared with HG group, the levels of inflammatory cytokines TNF-α, IL-1β, cell invasion ability, migration ability, the expression levels of N-cadherin, vimentin, FN and p-NF-κB p65 protein in Met group and Y group significantly decreased, and the expression level of E-cadherin protein increased (P<0.05). Compared with the Met group, the above indexes changed more significantly after the addition of BAY 11-7082 (P<0.05), and the trend of Met+A group was opposite to that of Met+Y group (P<0.05). Metformin inhibits D-glucose-induced HGPC inflammatory response, invasion, migration and epithelial-mesenchymal transition process by blocking the activation of NF-κB pathway, and protects podocyte injury induced by high glucose. Key words: diabetic nephropathy; metformin; nuclear factor-κB signaling pathway; D-glucose induction; inflammation of human glomerular podocytes （Acta Laser Biology Sinica, 2024, 33(3): 275-283） 2024 Vol. 33 (3): 275-283 [Abstract] ( 156 ) [HTML 1KB] [PDF 3428KB] ( 1091 ) 284 XU Chao, LI Zhen, MENG Di, LI Jun Effects of Oridonin on Migration and Invasion of Human Chondrosarcoma SW-1353 Cells (Department of Orthopaedics, Beidaihe Hospital of Qinhuangdao, Qinhuangdao 066100, China) Abstract: To investigate the effects of oridonin on migration and invasion of human chondrosarcoma cells. In vitro experiments, human chondrosarcoma SW-1353 cells were divided into control group being without intervention, 1.25, 2.50, 5.00 μmol/L oridonin groups (1.25, 2.50, 5.00 μmol/L oridonin) and positive drug group (6 μmol/L adriamycin). Human chondrosarcoma SW-1353 cells were tested for viability, mobility, invasion ability and epithelial-mesenchymal transition (EMT) expression. The results show that: compared with the control group, SW-1353 cell viability in the 5.00 μmol/L oridonin group and positive drug group significantly decreased (P<0.05). 1.25, 2.50, 5.00 μmol/L oridonin groups and positive drug obviously inhibted cell migration and invasion in a concentration-dependent manner (P<0.05). The expression of E-cadherin decreased the expression of vimentin and N-cadherin and inhibited EMT process by increasing the expression of E-cadherin protein, and the higher the concentration, the more significant the change was (P<0.05). Lamonidin can significantly inhibit the migration and invasion of human chondrosarcoma SW-1353 cells, or through mediating EMT, which provides a theoretical basis for the biological study of human chondrosarcoma cells. Key words: oridonin; chondrosarcoma; SW-1353; migration; invasion （Acta Laser Biology Sinica, 2024, 33(3): 284-288） 2024 Vol. 33 (3): 284-288 [Abstract] ( 167 ) [HTML 1KB] [PDF 2243KB] ( 1155 )

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