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Abstract (Department of Orthopaedics, Beidaihe Hospital of Qinhuangdao, Qinhuangdao 066100, China)
Abstract: To investigate the effects of oridonin on migration and invasion of human chondrosarcoma cells. In vitro experiments, human chondrosarcoma SW-1353 cells were divided into control group being without intervention, 1.25, 2.50, 5.00 μmol/L oridonin groups (1.25, 2.50, 5.00 μmol/L oridonin) and positive drug group (6 μmol/L adriamycin). Human chondrosarcoma SW-1353 cells were tested for viability, mobility, invasion ability and epithelial-mesenchymal transition (EMT) expression. The results show that: compared with the control group, SW-1353 cell viability in the 5.00 μmol/L oridonin group and positive drug group significantly decreased (P<0.05). 1.25, 2.50, 5.00 μmol/L oridonin groups and positive drug obviously inhibted cell migration and invasion in a concentration-dependent manner (P<0.05). The expression of E-cadherin decreased the expression of vimentin and N-cadherin and inhibited EMT process by increasing the expression of E-cadherin protein, and the higher the concentration, the more significant the change was (P<0.05). Lamonidin can significantly inhibit the migration and invasion of human chondrosarcoma SW-1353 cells, or through mediating EMT, which provides a theoretical basis for the biological study of human chondrosarcoma cells.
Key words: oridonin; chondrosarcoma; SW-1353; migration; invasion
（Acta Laser Biology Sinica, 2024, 33(3): 284-288）
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YANG Yue, WANG Hui, WANG Rui, MA Ke, XU Ying, ZHANG Shuijun, ZHANG Zhenzhen, CHEN Rong, MENG Qingguo, FENG Zhixin. Expression, Purification, and Preliminary Crystallographic Study of Ferritin from Spiroplasma eriocheiris#br#[J]. journal1, 2024, 33(3): 227-235. |
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