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Abstract (1. School of Biomedical Engineering, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230026, China; 2. Jiangsu Key Laboratory of Medical Optics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China)
Abstract: The choroid plexues in the zebrafish hindbrain controls the exchange of substances between brain and its vessels, and the vascular abnormalities around the choroid plexues can lead to associated cerebrovascular diseases. We first performed serial imaging of the major blood vessels in the hindbrain region of zebrafish, and obtained a series of images of zebrafish between 43 hours and 63 hours after fertilization using confocal microscopy to photograph embryos under normal conditions and after cylindrospermopsin treatment. Afterwards, the DE-3D U-Net was developed for the segmentation of the hindbrain images. The segmentation results showed that the class pixel accuracy of DE-3D U-Net could reach to 86.67%, 93.18% and 83.74% for the three main vessels and the segmentation results were used to plot vascularization curves. The quantitative results showed that the radii of the three vessels showed a reduction of 18% after treatment with high concentrations of cylindrospermopsin, and the angle of the mesencephalic vein showed a reduction of 30° to 50°. This study proposed a neural network for segmentation of hindbrain vessels of zebrafish, and it provided a basis for research on the development of zebrafish choroid plexsus.
Key words: zebrafish; hindbrain vessels; confocal microscopy; 3D U-Net deep-learning network; cylindrospermopsin
（Acta Laser Biology Sinica, 2024, 33(3): 209-216）
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