激光生物学报
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2023 Vol. 32 No. 2
Published: 2023-04-28
1
2023 Vol. 32 (2): 1-2 [
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KE Cheng, WU Chunfa, LI Weijun, ZHENG Jian, LI Min, WANG Min, HUANG Zheng
Rational Understanding of Blue Light Hazard and Blue Light Biological Safety Assessment
Abstract: Human being can be exposed to various blue light in daily life and workplace. The biological effects and biosafety of blue light have attracted more and more attentions in recent years. A series of guidelines, technical reports and standards have been issued at home and abroad on the potential health hazards of blue light, the biological safety assessment of blue light and the risk group of blue light products. The “blue light hazard” is a technical term used in the standard system for the photobiological safety assessment of lights and lamp systems. The term does not generically imply that blue light radiation is risky nor should it be mistakenly considered as harmful. Blue light plays an important role in maintaining normal vision and human health. Rational understanding of blue light hazard would have positive effects on the development and application of blue light technology. This article focuses on some important properties and sources of blue light radiation, biological effects of blue light and the assessment system of blue light biosafety, and intends to make an objective and rational analysis of the current consensus and misunderstanding on the blue light hazard, and reviews the relevant standards on the biosafety of blue light radiation and the evaluation methods of blue light biosafety.
Key words: blue light hazard; blue-light hazard function; biosafety of blue light; exposure limit; maximum permissible exposure duration
(Acta Laser Biology Sinica, 2023, 32(2): 097-110)
2023 Vol. 32 (2): 97-110 [
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XIAO Fuliang, ZHAI Xiuming, LI Jie, TANG Min, HOU Yuji
Research Progress on the Regulation of Light Quality on the Growth Development and Quality Formation of Tea Plant
Abstract: Tea is an important economic crop in China. The development of tea planting and processing plays an important role in promoting economic development, rural poverty reduction and rural revitalization. As an important factor of light, light quality directly affects the growth and quality of tea plant. Understanding the response of tea plant to light quality stimulus is of great significance to the efficient development of tea industry. Studies have shown that light quality has a complex superposition effect and dose effect on the growth and quality formation of tea plants. It produces different stimulation effects at different developmental stages of callus growth and differentiation, and has complex biological effects on post-harvest processing. This paper summarizes the physiological, biochemical and molecular response mechanisms of light quality on the growth and development, asexual reproduction and processing of tea plants, aiming to provide a theoretical basis for light quality to promote high-quality growth, efficient breeding and quality improvement of tea plants.
Key words: light quality; tea plant; growth and development; quality formation; asexual reproduction
(Acta Laser Biology Sinica, 2023, 32(2): 111-117)
2023 Vol. 32 (2): 111-117 [
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ZHANG Xi, WANG Rui, YU Zhichao, ZHANG Shuo, HUANG Yanqin
Research Progress of Palmitic Acid Induced Oxidative Stress and Endoplasmic Reticulum Stress on Islet β Cell
Abstract: Type 2 diabetes mellitus (T2DM) is mainly caused by defective insulin secretion and insulin resistance of islet β cells. Palmitic acid is one of the most abundant free fatty acids in the human body. When its content in the body is too high, it is easy to cause lipid metabolism disorder and induce islet β cells dysfunction and insulin resistance, which are closely related to the occurrence and development of T2DM. However, the specific mechanism is not precise. The function of islet β cells could be affected by oxidative stress and endoplasmic reticulum stress (ERS) induced by palmitic acid. It is also the critical pathway for destroying insulin signaling. Palmitic acid aggravates oxidative stress by increasing mitochondrial oxidation, diacyl glycerol-protein kinase C-nicotinamide adenine dinucleotide phosphate pathway, changing the normal function of the mitochondrial respiratory chain and inflammatory stimulation. It also affects the folding ability of the endoplasmic reticulum, destroys the intracellular protein transport pathway, upregulates unfolded protein response-related transcription factors, makes palmitoylation, degrades carboxypeptidase E, and reduces Ca2+ in the endoplasmic reticulum to promote ERS. Both promote islet β cells dysfunction and apoptosis, ultimately leading to the occurrence and development of T2DM. This paper reviewed the relationship between palmitic acid and islet β cells, the correlation between intracellular oxidative stress and ERS and the potential Chinese and Western medicine-targeted intervention drugs such as protein kinases R inhibitor, Ginsenoside Rg1 and Sanhuang Decoction. It would provide a novel idea for the clinical treatment of T2DM.
Key words: islet β cell; type 2 diabetes mellitus; palmitic acid; oxidative stress; endoplasmic reticulum stress
(Acta Laser Biology Sinica, 2023, 32(2): 118-125)
2023 Vol. 32 (2): 118-125 [
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PAN Qilin, LI Miaomiao, XIAO Mucang, AI Baoquan, XIONG Jianwen
An Experimental Study Based on HA Modified CoFe
2
O
4
-TiO
2
Nanoparticles by Photodynamic Therapy for HL60 Cells
in vitro
Abstract: In this study, CoFe2O4-TiO2 was prepared by hydrothermal method, in which CoFe2O4 was the core and TiO2 was the shell, and HA@CoFe2O4-TiO2 was obtained by hyaluronic acid modification. The physicochemical properties of the nanocomposites were characterized by scanning electron microscopy (SEM) imaging, energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), ultraviolet-visible absorption spectroscopy (UV-Vis) and Fourier transform infrared spectroscopy (FTIR). CCK-8 method was used to investigate the toxicity of HA@CoFe2O4-TiO2 nanocomposites on HL60 cells in dark chamber, and the inactivation effect of photodymanic therapy (PDT). The mechanism of interaction between CoFe2O4, hyaluronic acid and TiO2 on PDT inactivation of HL60 cells was preliminarily investigated by fluorescence spectra (FS), intracellular ROS production and uptake level analysis of nanocomposites. The experimental results showed that the morphology of HA@CoFe2O4-TiO2 was regular and spherical, and the size met the requirements of cell uptake. The visible response range of TiO2 was broadened with CoFe2O4. Dark toxicity experiment proved that HA@CoFe2O4-TiO2 nanocomposites were less toxic to HL60 cells under darkroom conditions. Meanwhile, Compared with the groups treated with TiO2 and CoFe2O4-TiO2 nanoparticles, the photocatalytic inactivation efficiency of the group treated with HA@CoFe2O4-TiO2 was as high as 82.6%. Therefore, it has the potential to become a photosensitizer for the PDT treatment of leukemia in vitro, improving photodynamic efficiency and targeting.
Key words: CoFe2O4-TiO2; hyaluronic acid; photodynamic therapy; visible light; HL60 cells
(Acta Laser Biology Sinica, 2023, 32(2): 126-138)
2023 Vol. 32 (2): 126-138 [
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LUO Sen, YANG Kun, LI Min, XIAO Wenxuan, LEI Wei, ZHANG Zixuan, CHEN Minzhi
Discovery and Identification of a Novel Nav1.7 Peptide Inhibitor from Spider Venom
Abstract: Voltage-gated sodium channel Nav1.7 is preferentially expressed in peripheral nociceptors. Clinical genetics,animal models and pharmacological studies indicate that Nav1.7 is an important target for analgesic drug development. In this paper,we report the isolation and characterization of APTX-1,a novel peptide toxin from the venom of spider Calommata signata Karsch. Patch-clamp analysis confirmed that APTX-1 potently inhibits the currents of Nav1.7. Mass spectrum results showed that the molecular weight of APTX-1 was 7 815.2 Da. The first 10 amino acid positions at the N terminal were ASCKQVGEEC. APTX-1 inhibited Nav1.7 currents in a concentration-dependent manner,with a half-inhibitory concentration of (0.46±0.08) μmol/L. Channel dynamics analysis showed that APTX-1 did not affect the reversal potential,voltage-dependent steady-state activation curve,and inactivation curve of Nav1.7,indicating that the peptide toxin did not affect the ion selectivity and voltage dependence of Nav1.7. In conclusion,we found a novel Nav1.7 peptide inhibitor,and the characteristics of its action on Nav1.7 were studied to provide lead molecules for the development of analgesic drugs targeting the Nav1.7.
Key words: Calommata signata Karsch; peptide toxin; Nav1.7; APTX-1; pain
(Acta Laser Biology Sinica, 2023, 32(2): 139-145)
2023 Vol. 32 (2): 139-145 [
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DU Jie, CAI Hailin, HUANG Binbin, HUANG Jun, ZHANG Cuiyang, LONG Qingshan,ZHANG Liang, CHEN Hairong, TANG Bingxuan, CHEN Wu, LIU Qingshu
Cloning, Prokaryotic Expression and Transcription Profile Analysis of
ede
B Gene in
Brevibacillus brevis
X23
Abstract: Brevibacillus brevis X23 can produce non-ribosomal antibiotic edeines, which have been widely used in biological control of plant diseases. The edeB gene in the edeine biosynthetic gene cluster regulates the synthesis of edeines. In this study, the prokaryotic expression vector pET28a-edeB was constructed by homologous recombination technology with edeB gene as the recombinant fragment of exogenous gene, and was transformed into Escherichia coli BL21(DE) for induced expression. The transcription profile of edeB gene in different culture times (12, 18, 24, 30 and 36 h) of B. brevis was detected by transcriptome sequencing. The results showed that the edeB gene was 771 bp in length, encoding 256 amino acids. SDS-PAGE electrophoresis showed that a specific band with a molecular weight of 30 kD appeared, which is in consistent with the bioinformatics prediction results. EdeB protein mainly existed in the form of inclusion bodies. The results of transcription profile showed that edeB gene was expressed at all culture times, and the gene expression showed a pattern of increasing at first and then decreasing with a peak at 30 h. These results laid a foundation for further study on the molecular mechanism of edeB gene regulating the synthesis of edeines, and provided a theoretical basis for the construction of high-yield edeine strains of B. brevis.
Key words: Brevibacillus brevis; edeB gene; prokaryotic expression; transcription profile; transcriptome
(Acta Laser Biology Sinica, 2023, 32(2): 146-152)
2023 Vol. 32 (2): 146-152 [
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HE Simei, ZHANG Yangjun, ZHANG Ke, LI Kunyan, XIA Liqiu, HU Shengbiao
The Diversity and Composition of Gut Microbiota in Six Species of Fish from the Dongting Lake Area
Abstract: The gut microbiota in fish is a natural immune barrier, which can inhibit the colonization and proliferation of pathogenic microorganisms, so as to protect the fish from diseases. Dongting Lake is a vital fish and aquatic organisms’ habitat and gene pool, with more Cypriniformes and Perciformes species present than Siluriformes. The fish community structure and biodiversity in Dongting Lake have attracted extensive attentions, whereas the ecosystem has been damaged to a certain extent. Few studies have taken the characteristics of fish intestinal microbiota as a potential factor to improve the ecosystem of Dongting Lake. In order to detect the microbial community in important fish populations in Dongting Lake. The 16S rRNA high-throughput sequencing was used to characterize the gut bacterial community diversity, composition and potential functions of six fishes including Pelteobagrus fulvidraco, Siniperca chuatsi, Carassius auratus, Culter alburnus, Ctenopharyngodom idella, Hypophthalmichthys molitrix, which are in Siluriformes, Perciformes and Cypriniformes. There were five dominant phyla in all fish gut samples, including Fimicutes, Proteobacteria, Fusobacteriota, Actinobacteriota and Cyanobacteria. The bacterial composition of Cypriniformes and Perciformes was similar. The proportion of Cetobacterium in Siluriformes was high, while the proportion of Cetobacterium and Aeromonas in Cypriniformes were high. The species composition and function of the microbial community of the gut microbiota of the six fish species were predicted. We found that the species richness of the gut microbiota of Siniperca chuatsi was the highest, and the metabolic process was more vigorous. We speculated that this may be related to the disease resistance and adaptability of Perciformes. An in-depth understanding of the diversity, composition and potential functions of fish gut microbiota can not only better regulate the use of probiotics and their water quality management, but also have important reference values for the healthy breeding of fish.
Key words: fish; gut microbiota; Cypriniformes; Perciformes; Siluriformes
(Acta Laser Biology Sinica, 2023, 32(2): 153-159)
2023 Vol. 32 (2): 153-159 [
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WANG Zhou, WANG Hongwei, WANG Ya, YANG Junfang, ZHAO Yiting, ZHANG Hongbin, CAO Yue
Gene Family Identification and Expression Analysis of CeSA Transcription Factor in Ricinus communis
Abstract: Cellulose synthase A (CeSA) is a protein that can synthesize cellulose on the plasma membrane of plant cells,and has cis-acting elements related to leaf morphological development. In the process of higher plant growth and development,the elongation of cells is affected by hormones and the environment,making plant growth highly different. Among them,the cell wall plays a limiting role in the elongation length of cells,while cellulose is an important component of the cell wall and has an important impact on the cell morphology of plants. CeSA is an important regulator of plant growth and development. In this study,the gene structure,chromosome localization,physicochemical properties,secondary structure,tertiary structure,signal peptide and subcellular localization,protein transmembrane domain,collinearity analysis,conserved Motifs,promoter homeopathic elements and phylogeny of CeSA family members were identified and analyzed by the identification and biogenetic informatics of the reference genome of Ricinus communis,combined with the castor genome database. A total of 8 CeSA transcription factors were identified from the castor reference genome,named RcCeSA1~RcCeSA8,distributed on 8 chromosomes. The 8 RcCeSAs proteins were all hydrophilic proteins,did not contain signal peptides,and the protein subcellular localization was mainly on chloroplasts and Golgi apparatus. 70% of the α-spiral and irregular curling in secondary structures; The gene conservation analysis of RcCeSAs showed that Motifs except Motif1 and Motif2 were all conserved Motifs in the RcCeSA family. The analysis of gene promoter cis-acting elements found that there were leaf morphological development and related elements involved in defense and stress response in the promoter regions of RcCeSA1,RcCeSA4 and RcCeSA7 transcription factors,which may be rapidly expressed under adverse conditions and exert their potential functions. Phylogenetic tree analysis showed that the RcCeSA family was more closely related to soybean and sunflower. The results of this study explored the molecular mechanism and function of CeSA-related transcription factors in the regulation of plant cellulose synthesis in castor beans,which laid a foundation for further study of leaf morphological differences and cellulose content and the expression of genes related to cellulose synthesis in castor beans,and laid a foundation for establishing the important role of plant morphology in actual production.
Key words: Ricinus communis; CeSA transcription factor; gene family; plant cellulose synthesis; bioinformatics
(Acta Laser Biology Sinica, 2023, 32(2): 160-169)
2023 Vol. 32 (2): 160-169 [
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CAI Jianan, LIU Bin, ZHANG Fenghua, CHEN Yuting, XIANG Yuqing, CHANG Haiyan*, FANG Fang
R848 Addition to Aluminium Hydroxide Enhances Immune Protection in Mice Immunized with Inactivated Influenza H7N9 Vaccine
Abstract: Inactivated influenza virus vaccines usually require adjuvants to enhance their immune protection. Aluminium hydroxide (Al ) adjuvant is commonly used as an adjuvant in human vaccines, mainly inducing Th2 immune response. In this study, Al adjuvant and TLR7/8 agonist R848 were combined to form a complex adjuvant, and its adjuvancity was evaluated in immunization of mice with inactivated influenza virus H7N9 vaccine. Three weeks after a single immunization, mice were attacked with a lethal dose (20 LD50) of homologous virus. The results showed that, compared with vaccine adjuvanted with R848 or Al alone, R848+ Al adjuvanted vaccine enhanced mice higher titers of serum IgG and IgG2a subtype antibodies and induced both Th1 and Th2 cytokines, with a Th1-biased response, and provided potent protection in mice against lethal virus infection. Therefore, the adjuvancity of R848+ Al is superior to that of R848 or Al alone. The study may help to improve Al adjuvancity and immune effects of inactivated influenza vaccines.
Key words: R848; aluminium hydroxide; inactivated vaccine; influenza virus
(Acta Laser Biology Sinica, 2023, 32(2): 170-176)
2023 Vol. 32 (2): 170-176 [
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NIE Ganga, QI Junhua
The Expression of
NME1
in Skin Cutaneous Melanoma and Its Correlation with Clinical Prognosis
Abstract: The purpose of this study is to investigate the expression of non-metastatic cell 1 (NME1) in skin cutaneous melanoma (SKCM) and its correlation with clinical prognosis. The expression of NME1 in SKCM was analyzed using GEPIA database and UALCAN database. GSCA database, GEPIA database and TIMER database were used to analyze the relationship between the expression of NME1 and the overall survival rate of SKCM patients. The mutation of NME1 in SKCM and its correlation with the overall survival rate of SKCM patients were analyzed through cBioPortal database. The proteins that may interact with NME1 were analyzed by STRING database, and their functions were enriched by Metascape database. The correlation between the expression of NME1 and immune cells infiltration level in SKCM was analyzed using TIMER database. The results showed that the expression of NME1 in SKCM was significantly higher than that in normal tissues. The high expression of NME1 was significantly related to the lower overall survival rate of metastatic SKCM patients. NME1 gene mutation has no significant correlation with the overall survival rate of SKCM patients. NME1 and its interacting proteins are mainly involved in ARF6 trafficking pathway, purine metabolism, regulation of cell development, DNA metabolic process and other biological processes. The high expression of NME1 in metastatic SKCM was significantly correlated with the lower infiltration level of CD4+T cell, CD8+T cell, neutrophil, macrophage and dendritic cell. This study shows that the high expression of NME1 is related to the lower level of immune infiltrating cells, and the overall survival rate in patients with metastatic SKCM. NME1 is expected to become a biomarker related to the infiltration of immune cells and prognosis of metastatic SKCM.
Key words: SKCM; NME1; immune infiltration; clinical prognosis
(Acta Laser Biology Sinica, 2023, 32(2): 177-183)
2023 Vol. 32 (2): 177-183 [
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FENG Ling, HUANG Zhaoxia, QIN Lele, HU Yushan, ZHANG Yongqin
A Study on the Mechanism of Chaihu Shugan Powder in the Teatment of Hepatic Hemangioma Based on Network Pharmacology and Molecular Docking
Abstract: Hepatic hemangioma is one of the common benign tumors, which is treated with Chaihu Shugan Powder in clinical, but its specific mechanism of action is still unknown. In this study, network pharmacology and molecular docking methods were used to obtain the information of key targets and components through the analysis of the compositional target network diagram, and molecular docking of key targets and components was carried out to obtain the corresponding molecular docking map and explore the mechanism of action. A total of 142 ingredients of Chaihu Shugan Powder were obtained, and 61 targets were intersections with hepatic hemangioma. There were 184 biological processes for gene ontology (GO) functional enrichment, which mainly involving transcription factor activity, DNA binding, and positive regulation of transcription. 138 signaling pathways were identified by Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis, which mainly involve PI3K-Akt signaling pathway, Hepatitis B signaling pathway, MAPK signaling pathway, and AGE-RAGE signaling pathway, etc. Molecular docking showed that AKT1 protein was stable with β-sitosterol, VEGFA protein was stable with quercetin and isorhamnetin, ESR1 and MAPK3 were stable with β-sitosterol and naringin, respectively. The mechanism of Chaihu Shugan Powder in the treatment of hepatic hemangioma may be related to inducing angiogenesis and protecting the activation of blood vessels and liver cells, in which active ingredients such as quercetin, isorhamnetin, β-sitosterol, kaempferol and naringin, through act on targets such as STAT3, AKT1, VEGFA, ESR1 and MAPK3, which has provided theoretical reference for clinical aplication.
Key words: Chaihu Shugan Powder; hepatic hemangioma; network pharmacology; molecular docking; signal pathway
(Acta Laser Biology Sinica, 2023, 32(2): 184-192)
2023 Vol. 32 (2): 184-192 [
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