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Abstract Abstract: Cellulose synthase A (CeSA) is a protein that can synthesize cellulose on the plasma membrane of plant cells，and has cis-acting elements related to leaf morphological development. In the process of higher plant growth and development，the elongation of cells is affected by hormones and the environment，making plant growth highly different. Among them，the cell wall plays a limiting role in the elongation length of cells，while cellulose is an important component of the cell wall and has an important impact on the cell morphology of plants. CeSA is an important regulator of plant growth and development. In this study，the gene structure，chromosome localization，physicochemical properties，secondary structure，tertiary structure，signal peptide and subcellular localization，protein transmembrane domain，collinearity analysis，conserved Motifs，promoter homeopathic elements and phylogeny of CeSA family members were identified and analyzed by the identification and biogenetic informatics of the reference genome of Ricinus communis，combined with the castor genome database. A total of 8 CeSA transcription factors were identified from the castor reference genome，named RcCeSA1～RcCeSA8，distributed on 8 chromosomes. The 8 RcCeSAs proteins were all hydrophilic proteins，did not contain signal peptides，and the protein subcellular localization was mainly on chloroplasts and Golgi apparatus. 70% of the α-spiral and irregular curling in secondary structures; The gene conservation analysis of RcCeSAs showed that Motifs except Motif1 and Motif2 were all conserved Motifs in the RcCeSA family. The analysis of gene promoter cis-acting elements found that there were leaf morphological development and related elements involved in defense and stress response in the promoter regions of RcCeSA1，RcCeSA4 and RcCeSA7 transcription factors，which may be rapidly expressed under adverse conditions and exert their potential functions. Phylogenetic tree analysis showed that the RcCeSA family was more closely related to soybean and sunflower. The results of this study explored the molecular mechanism and function of CeSA-related transcription factors in the regulation of plant cellulose synthesis in castor beans，which laid a foundation for further study of leaf morphological differences and cellulose content and the expression of genes related to cellulose synthesis in castor beans，and laid a foundation for establishing the important role of plant morphology in actual production.
Key words: Ricinus communis; CeSA transcription factor; gene family; plant cellulose synthesis; bioinformatics
（Acta Laser Biology Sinica, 2023, 32(2): 160-169）
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