Abstract：In order to investigate biological function of soybean Lea5 gene, one of LEA gene family, the overexpression vector of pBI121Lea5 was constructed. Through leaf discs transformation by Agrobacterium (LB4404:pBI121Lea5), four transgenic plants of TL06, TL09, TL17 and TL32 were selected and identified. Analysis by RTPCR shows that soybean Lea5 gene was expressed in T2 generation plants of the four transgenic lines in different level. Treated with drought and salinity, T2 generation plants of TL09 and TL32 displayed more resistance to drought and more tolerance to salinity. This indicates that overexpression of soybean Lea5 gene confers resistance to drought and tolerance to salinity in plant.
