Abstract：Dendrobium candidum has important medicinal value, but with limited resources, so it has great significance to its full development and utilization. In this study, the extraction of polysaccharide from Dendrobium candidum was assisted by αLrhamnosidase. The extraction rate of Dendrobium candidum polysaccharide was improved by optimizing three factors, including temperature, time and enzyme quantity. The results showed that the polysaccharide extraction rate reached the maximum (38.4%)at 40 ℃, 1 h with 2.5% enzyme quantity and 21.7% without enzyme treatment. At the same time, the effects of αLrhamnosidase on the volatile constituents of Dendrobium candidum were analyzed by HSSPMEGCMS. GCMS analysis indicated that 31 kinds of volatile components were found and the main constituents were hexanal, 1octen3ol, isomenthol and 3octen2one. The relative content of aldehydes in the sample were the highest and 6 kinds of new volatile components were produced after enzyme treatment. The results showed that αLrhamnosidase has a positive effect on the extraction rate of polysaccharide from Dendrobium candidum, which provides a basis for the research and application of Dendrobium candidum polysaccharides, also gives some references for the research and development of the volatile components of Dendrobium candidum
2017, Vol. 26 
