Abstract：Plant MAPK (mitogenactivated protein kinases) cascades play a crucial role in the process of hormone signal transduction, various stresses responses and plant growth and development. The fulllength cDNA sequence of Akebia trifoliata mapk3 gene was amplified by using the technology of rapid amplification of cDNA ends (RACE) method. Subsequently, characterization of Aktmapk3, including gene alignment, phylogenetic tree, conserved domain and protein structure were analyzed by bioinformatics, while the expression level of mapk3 in different tissues of Akebia trifoliata were analyzed by realtime quantitative PCR method. Result showed that the Aktmapk3 gene contained an 1 164 bp open reading frame (ORF) which encodes 387 amino acids, and the coding protein contained an ATP binding site (IPR017441), MAPK conserved site (IPR003527), and Serine/threonineprotein kinase active site (IPR008271) suggesting that this protein might be activated by phosphorylation, and then phosphorylate downstream protein to executive physiologic function. The result of Realtime PCR exhibited that Aktmapk3 was the highest abundance in bud, mature leaf and flower, suggested that Aktmapk3 might be involved in the development of bud and flower.
2017, Vol. 26 
