Abstract：Abstract: In order to study the regulation mechanism of gene expression in Drosophila heart development, obtaining the antibody of its corresponding target is essential, however so far there is no commercial antibodies used for research purpose, therefore we prepared antibodies rapidly by adopting DNA immune technology. Svp (seven-up) is one of the marker genes of cardiac development in Drosophila. In the first instance, Svp coding sequence which was obtained through PCR cloning was inserted into the pCAGGS-P7 eukaryotic expression vector. Secondly, we collected the antibodies by injecting recombinant vectors (pCAGGS-P7-Svp) directly into wild-type mice, making the exogenous genes expressed in the living body, through which antigens stimulated immune response and induces specific humoral and cellular immune response. Western blot and embryo immunofluorescence were used to detect the titer and specificity of Svp antibody. The result showed that polyclonal antibodies against Svp of high titer and specificity were obtained, which laid the foundation for further cardiac development research in Drosophila.
Key words: Svp; Drosophila; cardiac development; DNA vaccine; polyclonal antibody
引用本文:
李玉玲，赵梦婧，李瑞可，张亚楠，吴秀山，袁婺洲. 果蝇心脏发育标记基因Svp的多克隆抗体制备[J]. 激光生物学报, 2020, 29(5): 433-437.
LI Yuling, ZHAO Mengjing, LI Ruike, ZHANG Yanan, WU Xiushan, YUAN Wuzhou. Preparation of Polyclonal Antibodies against Svp, the Marker Gene of Cardiac Development of Drosophila. journal1, 2020, 29(5): 433-437.
