Synthetic Biology Applications for Enhancing the Efficient Production of Spinosad in Saccharopolyspora spinosa
(State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Provincial Key Laboratory for Microbial Molecular Biology, School of Life Science, Hunan Normal University, Hunan 410000, China)
Abstract：Abstract: Spinosad, a secondary metabolite produced by Saccharopolyspora spinosa, has garnered significant attention globally due to its environmentally friendly and broad-spectrum insecticidal properties. The unique structure of spinosad and its analog, butenyl-spinosyn, underlies their distinctive mode of action. To meet the demands of large-scale industrial production, research teams worldwide have been striving to enhance the yields of spinosad and butenyl-spinosyn, with remarkable achievements accomplished through the application of synthetic biology techniques. This review summarizes the research conducted domestically and internationally on the regulation of metabolic pathways at various levels using synthetic biology techniques to improve the production of spinosad and butenyl-spinosyn. It focuses on four key aspects: chassis cell modification and optimization, multi-omics analysis, genetic circuit modification, and metabolic network regulation. Furthermore, it provides an outlook on future research directions in this field, and provides a new research strategy to promote the biosynthesis of spinosad by synthetic biology technology.
Key words: spinosad; Saccharopolyspora spinosa; synthetic biology; gene editing; metabolic pathway regulation
（Acta Laser Biology Sinica, 2024, 33(5): 400-407）
引用本文:
方 静，穰 杰，夏立秋. 利用合成生物学技术促进刺糖多孢菌多杀菌素的高效合成[J]. 激光生物学报, 2024, 33(5): 400-407.
FANG Jing, RANG Jie, XIA Liqiu. Synthetic Biology Applications for Enhancing the Efficient Production of Spinosad in Saccharopolyspora spinosa. journal1, 2024, 33(5): 400-407.
