Abstract：(State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300350, China)
Abstract: The high porosity and customizable structure of metal-organic frameworks (MOFs) allow them to bind a wide range of biomolecules and have great potential as biosensors. MOFs can exhibit luminescence from metal nodes, ligands or introduced guests, which provides an excellent fluorescence response for sensing. However, single-signal emitting MOFs are susceptible to interference from concentration, environment, and excitation intensity, resulting in poor accuracy. Dual-fluorescence-emitting MOFs biosensors overcome these shortcomings and need to be fully and accurately characterized. Fluorescence resonance energy transfer (FRET) is the process in which the internal energy of fluorescent molecules is transferred from the donor to the acceptor, and FRET detection of dual-emission MOFs based on laser scanning confocal microscopy can achieve visual imaging and accurate efficiency analysis, and has become an effective evaluation method for dual-emission MOFs. In this paper, the technical process and conditions of FRET for the detection of dual-emission MOFs are described, and combined with the research examples of Alzheimer's disease and COVID-19, the application progress of MOFs fluorescent biosensors in terms of design concept, binding mode, and action efficiency is demonstrated. The results confirmed that this technique has high detection sensitivity and specificity, which provides a new idea for probe development and evaluation.
Key words: fluorescence resonance energy transfer; metal-organic frameworks; laser confocal microscopy; fluorescent
biosensors; double fluorescence emission
（Acta Laser Biology Sinica, 2024, 33(6): 481-488）
引用本文:
张 妍，肖楠楠. FRET表征双发射MOFs荧光生物传感器的研究进展[J]. 激光生物学报, 2024, 33(6): 481-488.
ZHANG Yan, XIAO Nannan. Research Progress of Dual-emission MOFs Fluorescent Biosensors by FRET. journal1, 2024, 33(6): 481-488.
