Abstract：Abstract: Microphthalmia-related transcription factors (MITF) are closely related to the formation of melanocytes (melanophores). The casper mutant zebrafish is a double-gene mutant zebrafish strain (mitfaw2/w2; roya9/a9) bred by crossing nacre and roy orbison. The observation of fin, scale and early body color development of mitfa-mutated casper zebrafish in this paper confirmed that there were no melanocytes in anal fin, dorsal fin and dorsal scale of casper zebrafish, and no melanocytes were generated during the early body color development. The recombinant plasmid of mitfa overexpression was constructed and microinjected into the fertilized eggs of casper zebrafish. Melanocytes were observed in the head, back and abdomen of the injected group. The results further confirmed that mitfa plays an important role in the formation of fish body color, especially in the formation of melanocytes. At the same time, the preparation of mitfa over-expressing casper zebrafish also provides a material platform for in-depth research on the molecular regulation of fish body color.
Key words: zebrafish; mitfa; melanophores; casper mutant; gene over-expression
（Acta Laser Biology Sinica, 2021, 30(5): 429-434）
引用本文:
张广婧，王 梅，刘利生，张永勤，彭亮跃，刘文彬，肖亚梅，刘锦辉. casper斑马鱼体色观察及过表达mitfa对其体色发育的影响[J]. 激光生物学报, 2021, 30(5): 429-434.
ZHANG Guangjing, WANG Mei, LIU Lisheng, ZHANG Yongqin, PENG Liangyue, LIU Wenbin, XIAO Yamei, LIU Jinhui. Observation of the Body Color of Zebrafish casper Mutant and the Effect of Overexpression of mitfa on Its Body Color. journal1, 2021, 30(5): 429-434.
