Abstract:Objective:The aim is to establish regeneration system in vitro of Phytolacca acinosa Roxb. Methods: To screen regeneration system scheme of Phytolacca acinosa Roxb, young stem and stem node and leaves and petioles and apical buds of Phytolacca acinosa Roxb were chosen as explants, and MS was used as basic medium with different plant growth regulators combinations to induce callus and buds and roots. Results: Callus could be induced from apical buds and young stem with early emergence and high quality, and the callus induction rate reached 100% in medium MS+6-BA 0.5 mg/L+ 2.4-D 0.5 mg/L. Among them, only the callus produced from apical bud could differentiate cluster buds. The medium for bud differentiation was MS+6-BA 2.0 mg/L+NAA 0.25 mg/L, and the induction rate reached 98%. The suitable medium for rooting was 1/2 MS + NAA 0.3 mg/L with 100% induction rate. Conclusion:The regeneration system in vitro of Phytolacca acinosa Roxb was established and perfected, which laid a foundation for the construction of genetic transformation system in Phytolacca acinosa Roxb.
引用本文:
邓雯韬,田〓宇,谢〓琴,肖英粟,苏〓益,蔺万煌. 商陆离体再生体系的构建[J]. 激光生物学报, 2018, 27(3): 275-280.
DENG Wentao, TIAN Yu, XIE Qin, XIAO Yingsu, SU Yi, LIN Wanhuang. Establishment of Regeneration System in vitro of Phytolacca acinosa Roxb. journal1, 2018, 27(3): 275-280.