Abstract:ASB11 is involved in the development of embryonic neural progenitor cells, regenerative myogenesis and ubiquitination, but its detailed mechanism of action has not been known. In order to further analyze the mechanism of zebrafish Asb11 gene, the antiASB11 specific polyclonal antibody by immunizing mice with DNA was prepared. The eukaryotic expression vector pCAGGSP7/ASB11 was constructed by using cDNA of zebrafish Asb11. BALB/c mice of 68 weeks old were immunized with pCAGGSP7/ASB11 to prepare antiserum against ASB11. The results showed that the pCAGGSP7/ASB11 recombinant plasmid was constructed with a rather good immunogenicity. The antibody valence of antiserum obtained from the immunized mice was 1〖DK〗∶400. Western blotting and indirect immunofluorescence showed that the antiserum prepared by immunizing mice with DNA could specifically recognize the ASB11 protein. This research established afoundation to reveal the mechanism of ASB11 protein.
引用本文:
蔡英桂,尹丽阳,王宏波,陈〓宇,曾〓蓉,吴秀山,叶湘漓. 利用DNA免疫技术制备ASB11蛋白的多克隆抗体[J]. 激光生物学报, 2018, 27(3): 260-264.
CAI Yinggui, YIN Liyang, WANG Hongbo, CHEN Yu, ZENG Rong, WU Xiushan, YE Xiangli. Preparation of Polyclonal Antibody Against ASB11 Protein by DNA Immunization. journal1, 2018, 27(3): 260-264.