Abstract:Macrophage polarization plays an important role in bone repair. Once being implanted in the body, bone repair materials will trigger response of macrophage, making it switch to M1/M2 phenotype, thus affect the osteogenic effect of the material. In this work, Strontiumsubstituted micro/nano bioactive glasses (SrMNBG)with 0, 5%, 10% and 15% molar percent of strontium element were prepared, and the effect of SrMNBG on macrophage polarization was studied. Subsequently, the effects of SrMNBG with different strontium incorporation ratio on the osteogenic differentiation of murine bone marrow mesenchymal stem cells (mMSCs) were evaluated. Finally, the in vivo assessment was conducted to verify the polarization of macrophage and osteogenesis of SrMNBG through in situ bone defect repair experiment. The in vitro results showed that compared with 0%SrMNBG,SrMNBG with 5%, 10% and 15% molar percent of strontium element could further promote macrophage polarization toward M2, and 10%SrMNBG displayed the best effect. Moreover, the secretion of macrophages modulated by 10%SrMNBG was more beneficial to osteogenesis. The in vivo results showed that there were more M2 macrophages around 10%SrMNBG after implantation in the body, and better bone repair effect was found in the 10%SrMNBG group, which were consistent with the in vitro results. Therefore, SrMNBG is expected to be a promising immunoregulatory material for bone repair.
引用本文:
张〓文,黄德球,郭周义,陈晓峰. 掺锶生物活性玻璃通过调控巨噬细胞极化促进成骨[J]. 激光生物学报, 2018, 27(3): 232-239.
ZHANG Wen, HUANG Deqiu, GUO Zhouyi, CHEN Xiaofeng. Enhanced Osteogenesis of Strontiumsubstituted Bioactive Glasses through Regulation of Macrophage Polarization. journal1, 2018, 27(3): 232-239.