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Quick Search     Advanced Search   2024 Vol.  33 No.  6 Published: 2024-12-28 1 2024 Vol. 33 (6): 1-2 [Abstract] ( 43 ) [HTML 1KB] [PDF 783KB] ( 139 ) 1 2024 Vol. 33 (6): 1-6 [Abstract] ( 34 ) [HTML 1KB] [PDF 21548KB] ( 146 ) 481 ZHANG Yan, XIAO Nannan Research Progress of Dual-emission MOFs Fluorescent Biosensors by FRET (State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300350, China) Abstract: The high porosity and customizable structure of metal-organic frameworks (MOFs) allow them to bind a wide range of biomolecules and have great potential as biosensors. MOFs can exhibit luminescence from metal nodes, ligands or introduced guests, which provides an excellent fluorescence response for sensing. However, single-signal emitting MOFs are susceptible to interference from concentration, environment, and excitation intensity, resulting in poor accuracy. Dual-fluorescence-emitting MOFs biosensors overcome these shortcomings and need to be fully and accurately characterized. Fluorescence resonance energy transfer (FRET) is the process in which the internal energy of fluorescent molecules is transferred from the donor to the acceptor, and FRET detection of dual-emission MOFs based on laser scanning confocal microscopy can achieve visual imaging and accurate efficiency analysis, and has become an effective evaluation method for dual-emission MOFs. In this paper, the technical process and conditions of FRET for the detection of dual-emission MOFs are described, and combined with the research examples of Alzheimer's disease and COVID-19, the application progress of MOFs fluorescent biosensors in terms of design concept, binding mode, and action efficiency is demonstrated. The results confirmed that this technique has high detection sensitivity and specificity, which provides a new idea for probe development and evaluation. Key words: fluorescence resonance energy transfer; metal-organic frameworks; laser confocal microscopy; fluorescent biosensors; double fluorescence emission （Acta Laser Biology Sinica, 2024, 33(6): 481-488） 2024 Vol. 33 (6): 481-488 [Abstract] ( 47 ) [HTML 1KB] [PDF 2579KB] ( 135 ) 489 BAO Wuxia, SONG Kai, PENG Yinghong, XU Ping, HE Ya-Wen Research Progress in Synechococcus sp. PCC 11901: A Chassis Cell in Carbon-negative Synthetic Biology (1. Carbon Negative Synthetic Biology for Biomaterial Production from CO2, Campus for Research Excellence and Technological Enterprise, SJTU Asia-Pacific Graduate Institute, Singapore 138602, Singapore; 2. State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China) Abstract: Carbon-negative synthetic biology aims to directly capture CO2 to produce bio-products. This technique not only solves the “food vs. fuel” dilemma in traditional biological production, but also achieves CO2 peaking and carbon neutrality, therefore, it has obvious economic, ecological, and social benefits. Cyanobacteria, which use light energy to convert CO2 into biomass, are ideal chassis cells in CNSB. The newly discovered Synechococcus sp. PCC 11901 is a robust cyanobacterial strain with high resistance to salt and light, fast growth and big biomass, and multiple genetic toolbox available, representing a rising star among the cyanobacterial chassis cells. This review summarized the research progress in PCC 11901, including its morphology, biochemical and physiological features, genomic characteristics, available genetic toolboxes, and bio-products derived from engineered PCC 11901, with the aim of providing theoretical references for future research related to PCC 11901. Key words: cyanobacterium; Synechococcus sp. PCC 11901; growth and biomass; genetic manipulation; carbon-negative synthetic biology （Acta Laser Biology Sinica, 2024, 33(6): 489-495） 2024 Vol. 33 (6): 489-495 [Abstract] ( 42 ) [HTML 1KB] [PDF 2416KB] ( 120 ) 496 HE Guangzu, YANG Hua, YE Huihui, LI Ye, LI Fengzhen, ZOU Zhenxing, WANG Bin, HU Zhan The Composition and Research Methods of Rumen Microbiota  in Ruminants (1. Hunan Polytechnic of Environment and Biology, Hengyang 421005, China; 2. Hunan Institute of Microbiology, Changsha 410009, China) Abstract: The study of rumen microbiota is of great significance for understanding the health, growth and development, as well as digestion and absorption processes of ruminants. In recent years, with the development of high-throughput sequencing technology and the rise of microbiome research, significant progress has been made in the study of rumen microbiota in ruminants. This article mainly reviews the composition and influencing factors of rumen microbiota in ruminants, as well as the technical methods for studying rumen microbiota, including 16S rRNA gene sequencing, functional genomics analysis, metabolomics research, and fluorescence in situ hybridization (FISH). Meanwhile, the application and development prospects of these methods in the study of rumen microbiota in ruminants will also be explored, aiming to provide reference for the structural and functional analysis of rumen microbiota. Key words: ruminant animals; rumen microbiota; high throughput sequencing; microbial community; multiomics joint analysis （Acta Laser Biology Sinica, 2024, 33(6): 496-504） 2024 Vol. 33 (6): 496-504 [Abstract] ( 54 ) [HTML 1KB] [PDF 1931KB] ( 125 ) 505 LI Guangfu, LYU Leili Application of Internet of Things Technology Based on RFID/Laser Scanning in Clinical Trials (College of Management, Gansu Agricultural University, Lanzhou 730070, China) Abstract: This paper aims to explore the application of radio frequency identification (RFID) and laser scanning based internet of things (IoT) technology in clinical trials, and analyze its advantages in improving data accuracy, improving work efficiency, and ensuring data security. RFID technology realizes the automatic identification and collection of items and data through radio waves, which has the characteristics of no contact reading, high capacity data storage and real-time information update. Laser scanning technology uses a laser beam to scan a bar code or quick response (QR) code to obtain information, with high precision and high speed. Both techniques performed well for subject identity verification, sample management, and medication tracking. In specific applications, RFID and laser scanning technologies have greatly improved the transparent management and traceability of trial materials, ensuring the real-time collection and monitoring of subject data, and safe distribution and use of drugs. These techniques reduce human error and improve data accuracy and work efficiency by automating and transmitting information in real time. Despite the challenges of technology costs, privacy protection, and standardization, these issues are expected to be gradually resolved with technological progress and innovation. In the future, RFID and laser scanning technology will be further integrated with emerging technologies such as artificial intelligence and blockchain to promote the digital and refined management of clinical trials. The research and analysis in this paper provide a reference for the digital transformation of clinical trials, hoping to promote the wide application of RFID and laser scanning technology in this field, and contribute to the innovation and progress of medical research. Key words: radio frequency identification; laser scanning; automatic recognition; internet of things technology; clinical trial （Acta Laser Biology Sinica, 2024, 33(6): 505-511） 2024 Vol. 33 (6): 505-511 [Abstract] ( 40 ) [HTML 1KB] [PDF 1662KB] ( 126 ) 512 WEI Haoyue, ZHANG Xi, WEI Daihao, LI Huan, WANG Rui, HUANG Yanqin HIF-1α/PFKFB3 Signaling Pathway Mediates GK Regulation of Islets in Type 2 Diabetes β Progress in Cellular Function Research (1. The First Clinical Medical College, Shandong University of Traditional Chinese Medicine, Jinan 250014, China; 2. College of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, China; 3. Weifang Hospital of Traditional Chinese Medicine, Weifang 261000, China; 4. Yanqing Hospital of Beijing Chinese Medicine Hospital, Beijing 102100, China; 5. Affiliated Hospital of Shandong University of Chinese Medicine, Jinan 250011, China) Abstract: Islet β cells are the exclusive cellular source of insulin synthesis and secretion, playing a crucial role in regulating blood glucose homeostasis. The impairment of islet β cells due to sustained anaerobic glycolysis pressure represents a significant factor contributing to insulin deficiency or resistance in patients with type 2 diabetes mellitus (T2DM). Glucokinase (GK), serving as the principal rate-limiting step in glucose transport, exhibits close association with insulin secretion. Hypoxia-inducible factor 1α subunit (HIF-1α) and 6-phosphofructo-2-kinase (PFKFB3) act as pivotal regulatory factors within glycolysis. The HIF-1α/PFKFB3 signaling pathway participates in intricate pathophysiological processes involving islet β cell function and maintenance under high-glucose conditions. This review elucidates the mechanisms by which the HIF-1α/PFKFB3 signaling pathway mediates GK induction through anaerobic glycolysis, mitochondrial network adaptive fragmentation, oxidative stress, and dedifferentiation leading to pancreatic islet β cell dysfunction in T2DM, thereby offering novel insights for clinical prevention and treatment strategies targeting T2DM. Key words: type 2 diabetes; glucokinase; HIF-1α/PFKFB3 signaling pathway; mitochondria; dedifferentiation （Acta Laser Biology Sinica, 2024, 33(6): 512-522） 2024 Vol. 33 (6): 512-522 [Abstract] ( 57 ) [HTML 1KB] [PDF 2110KB] ( 131 ) 523 ZHANG Xiuying, YANG Yang, ZHAO Yingchun A Dark-field Microscopy Imaging System Based on Two-lens (1. School of Flexible Electronics (Future Technologies), Nanjing Tech University, Nanjing 211816, China; 2. Shanghai AVIC Optoelectronics Co., Ltd, Shanghai 200245, China) Abstract: To address the challenge of characterizing the morphology of large transparent and unstained samples that traditional bright-field microscopy cannot solve, a dark-field imaging system based on two-lens is proposed. The system primarily consists of a laser, a spatial light modulator, two lenses, and a charge-coupled device (CCD) camera. Ring-shaped vortex light modulated by the spatial light modulator serves as the illumination, focused onto the sample surface by the front lens. After illumination, the scattered light carrying high-frequency sample information was collected by the rear lens for dark-field imaging of the object. Experimental comparisons were conducted using sections of dicotyledonous wood and pumpkin stems as samples. The results indicated that a self-built dark-field microscopy system based on two-lens can enhance imaging resolution and contrast, offering a valuable approach for morphology characterization of large transparent and unstained samples. Key words: two lens; vortex beam; dark field imaging; biological samples; morphological characterization （Acta Laser Biology Sinica, 2024, 33(6): 523-529） 2024 Vol. 33 (6): 523-529 [Abstract] ( 46 ) [HTML 1KB] [PDF 4896KB] ( 139 ) 530 PENG Jinfen, LIU Yong, TAN Zhiliang, ZHOU Chuanshe Effects of Laminaria japonica Extract Supplementation on Lactation Performance, Antioxidant Status, Immunity, Rumen Fermentation, and Rumen Microbiota in Dairy Cows (1. Institute of Subtropical Agriculture, Chinese Academy of Sciences, Key Laboratory for Agro-ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Changsha 410125, China; 2. University of Chinese Academy of Sciences, Beijing 100049, China) Abstract: The present study aimed to evaluate the effect of Laminaria japonica extract (LJE) supplementation on lactation performance, plasma biochemistry, immunity, antioxidant status, rumen fermentation parameters and rumen microbiota of dairy cows. The experiment was conducted in a one-way completely randomized design. 32 healthy lactating Holstein dairy cows with similar body weight, milk yield, parity and days in milk were selected and randomly assigned to 2 groups of 16 cows each. The cows in the control group (CON group) fed with a basal diet, and the other group (LJE group) received the CON diet supplemented with LJE at 30 g per cow per day. The entire trial includes a 12-day adaptation and a 30-day trial period. Compared with the CON group, the diet supplemented with LJE increased dry matter intake (DMI), milk yield, and milk protein content of dairy cows, whereas it had no significant effects on milk fat, lactose, somatic cell counts, and total solids, and it had no significant effects on the plasma biochemical indices. Moreover, the LJE group had significantly higher levels of immunoglobulin A (IgA), immunoglobulin G (IgG), and immunoglobulin M (IgM) in plasma, and had lower malondialdehyde content than those of the CON group. The total volatile fatty acid level increased in the rumen of dairy cows. The LJE diet increased the relative abundance of the phylum Firmicutes and the genera Succiniclasticum, Sphaerochaeta, Mucispirillum, and Akkermansia. In conclusion, adding LJE in feed can enhance the immunity of dairy cows, and reduce the level of lipid peroxidation in dairy cows, and improve the DMI, milk yield, and milk quality, which is of great significance to realize the healthy breeding of dairy cows. Key words: Laminaria japonica extract; milk performance; immunity; rumen fermentation; rumen microbiota （Acta Laser Biology Sinica, 2024, 33(6): 530-540） 2024 Vol. 33 (6): 530-540 [Abstract] ( 49 ) [HTML 1KB] [PDF 3434KB] ( 127 ) 541 LUO Haiyong, PANG Weiwei, LU Xuan, YANG Junxiao, TAN Lijun A Study on the Casual Relationship Between RETN Gene and BMD and Fracture (1. Hunan Institute of Traffic Engineering, Hengyang 421000, China; 2. Xiangxing College, Hunan University of TCM, Xiangyin 410007, China; 3. College of Life Science, Hunan Normal University, Changsha 410081, China; 4. Department of Orthopedics, Xiangya Hospital, Central South University, Changsha 410008, China) Abstract: Osteoporosis is the most common systemic and metabolic bone disease. The concentration of serum resistin (RETN) is associated with bone mineral density, but it is still unclear whether there is a causal relationship between RETN and bone density and fractures. This study used Mendelian randomization (MR) to analyze the causal relationship between RETN gene expression and bone mineral density and fractures, and then analyzed the differential expression of RETN gene in single-cell sequencing data of femeral neck fracture (FNF) and osteoarthritis (OA) samples. MR analysis showed that high expression of RETN was found to lead to decreased bone mineral density and increased risk of fractures. RETN expression was upregulated in the FNF group, and RETN was mainly expressed by neutrophils. The inflammatory chemokine CXCL8 related to RETN was significantly upregulated in the FNF group. This study suggested the role of RETN in the pathogenesis of osteoporosis, providing a basis for the diagnosis and treatment of osteoporosis. Key words: osteoporosis; RETN; Mendelian randomization; single-cell RNA sequencing; bone mineral density （Acta Laser Biology Sinica, 2024, 33(6): 541-549） 2024 Vol. 33 (6): 541-549 [Abstract] ( 50 ) [HTML 1KB] [PDF 2956KB] ( 138 ) 550 LI Wenqing, LI Li Empagliflozin Alleviates Acute Pancreatitis by Inhibiting Apoptosis in Rats (Affiliated Hospital of Xuzhou Medical University, Xuzhou 221000, China) Abstract: Acute pancreatitis（AP） is one of the most common gastrointestinal diseases requiring urgent hospitalization, and its incidence is on the rise. This study aims to evaluate whether empagliflozin (EMPA) can alleviate AP in rats by inhibiting apoptosis. Eighteen SPF-grade SD male rats were randomly divided into three groups using a random number table method: control group (CON group), AP group, and AP+EMPA group, with six rats in each group. After successful establishment of the rat models, blood and pancreatic samples were collected. The serum markers tested included amylase (AMY), glucose (GLU), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) levels. Western blot analysis was used to detect the expression levels of Caspase-3 and Bcl2 in the pancreatic tissue, while immunohistochemistry and immunofluorescence were used to assess Caspase-3 expression in the pancreatic tissue. Pancreatic tissues were paraffin-embedded for hematoxylin-eosin (HE) staining and pathological scoring. One-way ANOVA was employed for statistical analysis of the data. The study found that the AP group had elevated serum AMY, GLU, inflammatory cytokine levels, and pathological scores compared to the CON group. In the pancreatic tissue, the apoptosis-related molecule Caspase-3 was elevated, while the expression level of Bcl2 was reduced compared to the CON group (P<0.05). However, EMPA treatment could reverse these changes. In conclusion, EMPA effectively ameliorates the pathological changes in the pancreatic tissue in a rat model of AP, and this protective effect may be related to the inhibition of apoptosis. This article aims to explore the potential therapeutic effects and molecular mechanisms of EMPA in AP, providing new insights for the effective prevention and treatment of this disease. Key words: acute pancreatitis; apoptosis; Caspase-3; empagliflozin; rat model （Acta Laser Biology Sinica, 2024, 33(6): 550-556） 2024 Vol. 33 (6): 550-556 [Abstract] ( 46 ) [HTML 1KB] [PDF 3155KB] ( 128 ) 557 TAN Yuanyuan, LI Linghuan, YANG Yanqun, LIU Ling, WANG Xinyi, LIAO Beiya, XIONG Lei, XIE Huaping Construction and Effect on Spinal Development of hoxc8a Gene Knockout Line in Zebrafish (1. Laboratory of Animal Nutrition and Human Health, Hunan Normal University, Changsha, 410081, China; 2. Chenzhou Science and Technology Innovation Service Center, Chenzhou 423000, China) Abstract: The hoxc8a gene is a member of the Hox gene family, which is evolutionarily highly conserved. It has been studied that the hoxc8a gene is expressed in the swim bladder and pectoral fins of zebrafish, and that Hox genes can affect the formation and development of vertebrate body axes, whereas the mechanism of action of the hoxc8a gene is not clear.  In order to study the developmental biology functions and its molecular regulation mechanism of this gene, the gene will be knocked out using CRISPR/Cas9 gene editing technology. First, target sites as well as genotype detection primers were designed on exon 1 of the hoxc8a gene, and guide DNA (sgDNA) was obtained by PCR. Then the sgDNA was recovered and purified, and the purified sgDNA was used as a template to synthesize guide RNA (sgRNA) by in vitro transcription, and finally, RNA was purified. The sgRNA and Cas9 protein were mixed in a 1:1:0.8 ratio, and the mixture was injected into wild-type zebrafish embryos using microinjection technique, and the mutant F0 generation zebrafish were screened by genotyping. When the F0 generation zebrafish were sexually mature, they were crossed with wild-type zebrafish, and the offsprings were genotyped and screened to obtain the mutant heterozygotes of the F1, and the F1 generation of zebrafish with the protein code-shifting mutation were raised after Sanger sequencing, and continued to be bred to adult. The F1 zebrafish were crossed, and the offsprings were genotyped and screened to obtain F2 homozygotes. Finally, a calcium staining test was performed on mutant homozygous and wild-type zebrafish, and it was found that the spine development of the knockout mutant was not significantly different from that of the wild-type, suggesting that the hoxc8a gene does not affect the early spine development of zebrafish, whereas whether the gene affects the development of other tissues and organs remains to be further investigated. Thus, by constructing zebrafish hoxc8a knockout lines, thus we can provide a research basis for more in-depth studies of the hoxc8a gene. Key words: zebrafish; hoxc8a; spinal development; gene knockout line; homozygous mutant （Acta Laser Biology Sinica, 2024, 33(6): 557-566） 2024 Vol. 33 (6): 557-566 [Abstract] ( 49 ) [HTML 1KB] [PDF 3221KB] ( 138 ) 567 WANG Xiaoying, HAN Zhimei, LI Chunyan The Inhibition of Wnt/β-catenin Signal Pathway by Luteic Acid Alleviates the Damage and Apoptosis of Periodontal Membrane Cells （Department of Stomatology, Hebei Petro China Central Hospital, Langfang 065000, China） Abstract: To investigate the effects of luteic acid on inflammation and apoptosis of human periodontal stem cells induced by lipopolysaccharide and the regulation of Wnt/β-catenin signaling pathway, hPDLSCs were cultured in vitro, which was divided into two steps: pre-experiment and formal experiment. The pre-experiment was divided into control group (no intervention), lipopolysaccharide group (10 μg/mL lipopolysaccharide), low/medium/high concentration luboflavic acid group (10 μg/mL lipopolysaccharide +0.5, 1.0, 2.0 μmol/L luboflavic acid). The formal experiment was divided into control group, lipopolypaccharide group, luteic acid group (10 μg/mL lipopolypaccharide +2.0 μmol/L luteic acid), inhibitor group (10 μg/mL lipopolypaccharide +2 μmol/L Wnt/β-catenin signaling pathway inhibitor XAV939), luteic acid + inhibitor group (10 μg/mL lipopolysaccharide +2.0 μmol/L luteic acid +2 μmol/L XAV939) and luteic acid + activator group (10 μg/mL lipopolysaccharide +2.0 μmol/L luteic acid +20 μmol/L Wnt/β-catenin signaling pathway activator SKL2001), they were treated for 24 h. Cell viability was measured by CCK-8. The expression levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) were detected by enzyme-linked immunosorbent assay (ELISA). The apoptosis rate was detected by Hoechst 33258 staining. The expression levels of B lymphoblastoma-2 (Bcl-2), cysteine aspartate protease-3 (Caspase-3) and Wnt/β-catenin signaling pathway were detected by Western blot (Wb). In the preliminary experiment, the cell viability of LPS group decreased compared with control group (P<0.05), and the expression levels of inflammatory factors TNF-α, IL-1β and IL-6 increased (P<0.05). Compared with LPS group, the cell viability of high-concentration luteic acid group increased (P<0.05), and the levels of inflammatory factors TNF-α, IL-1β and IL-6 decreased (P<0.05). Therefore, the high-concentration luteic acid group was selected as the optimal concentration for formal experiment. In the formal experiment, the cell viability and anti-apoptotic Bcl-2 protein expression levels in LPS group decreased compared with those in control group (P<0.05), while the expression levels of inflammatory factors TNF-α, IL-1β and IL-6, apoptosis rate and the expression levels of Caspase-3, Wnt and β-catenin proteins increased (P<0.05). Compared with LPS group, the cell viability and anti-apoptotic Bcl-2 protein expression levels in luteic acid group and inhibitor group increased (P<0.05), while the expression levels of inflammatory factors TNF-α, IL-1β, IL-6, cell apoptosis rate and the expression levels of Caspase-3, Wnt and β-catenin proteins decreased (P<0.05). Compared with the luteic acid group, the change trend of the above indexes in the luteic acid + inhibitor group was more significant (P<0.05), and the change trend of these indexes in the luteic acid + activator group was significantly reversed (P<0.05). Luteic acid may promote the cellular activity of human hPDLSCs induced by lipopolysaccharide and inhibit their apoptosis and inflammation by inhibiting the signal transduction of Wnt/β-catenin signaling pathway. This study can provide a new theoretical reference for the treatment of periodontitis. Key words: periodontitis; gambogic acid; lipopolysaccharide; periodontal membrane stem cells; Wnt/β-catenin signaling pathway （Acta Laser Biology Sinica, 2024, 33(6): 567-576） 2024 Vol. 33 (6): 567-576 [Abstract] ( 40 ) [HTML 1KB] [PDF 4899KB] ( 121 )

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