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| The Two-component System YvrG/YvrH Regulates the Biosynthesis of Edeine |
| (1. Hunan Agricultural University a. College of Plant Protection; b. College of Agronomy, Changsha 410128, China; 2. Hunan Tobacco Science Research Institute, Changsha 410004, China; 3. Changsha Branch of Hunan Provincial Tobacco Company, Changsha 410011, China; 4. China Tobacco Hunan Industrial Co., LTD, Changsha 410014, China; 5. Hunan Institute of Microbiology, Changsha 410009, China) |
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Abstract Abstract: Edeine, synthesized by Brevibacillus brevis X23, is a linear peptide antibiotic not encoded by ribosomal RNA, renowned for its extensive range of antibacterial effectiveness. Transcriptome sequencing revealed an inverse expression pattern between the two-component regulatory factors yvrG/yvrH and the edeine biosynthesis gene cluster (ede BGC), suggesting the involvement of YvrH/YvrG in the transcriptional regulation of ede BGC. This study utilized Red/ET homologous recombination technology to construct X23 mutant strains that yvrG/yvrH lacks, complemented strains, and overexpressing strains, and investigated the impact of YvrG/YvrH on edeine biosynthesis through phenotype analysis and quantitative real-time PCR (RT-qPCR) techniques. The results revealed that knocking out yvrG/yvrH significantly reduced the antibacterial activity of the strains, the expression of ede BGC decreased, the synthesis of edeine was inhibited, and the yield of edeine was reduced by 46.75%. In summary, YvrG/YvrH act as positive regulatory factors in the edeine biosynthesis. This study provides gene regulatory elements for the metabolic engineering of edeine.
Key words: Brevibacillus brevis; edeine; two-component regulatory factors; bacteriostatic activity; biosynthesis
（Acta Laser Biology Sinica, 2024, 33(5): 461-469）
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