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Abstract Abstract: By using phospholipid molecules with hydrophilic and hydrophobic properties as matrix materials, the nanocomposite liposomes (Lip@Res/VC) containing both fat-soluble resveratrol (Res) and water-soluble vitamin C (VC) have been prepared through thin film hydration method and have been further used for cellular antioxidant studies. In the preparation of Lip@Res/VC, the optimal added amount of Res and VC were determined by evaluating the encapsulation rate of Res and VC, hydration particle size, polydispersal index and zeta potential of nanocomposite liposomes. The results showed that the hydration particle size of Lip@Res/VC was (92.29±5.41) nm and the encapsulation efficiency of Res and VC were (85.13±5.37)% and (64.73±5.51)%, when the added amount of Res was 4.0 mg and VC was 3.5 mg, respectively. The DPPH (2, 2-diphenyl-1-picrylhydrazyl) and ABTS ［2, 2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt］ free radical scavenging assay in vitro showed that the antioxidant activity of Lip@Res/VC was significantly higher than that of Lip@Res or Lip@VC. Furthermore, L02 cells were selected as model cells to study the intracellular antioxidant effect of Lip@Res/VC. The results showed that Lip@Res/VC is capable of removing intracellular reactive oxygen species (ROS) and protecting L02 cells from oxidative damage induced by hydrogen peroxide.
Key words: nanocomposite liposomes; resveratrol; vitamin C; reactive oxygen species; antioxidant
（Acta Laser Biology Sinica, 2023, 32(3): 226-233）
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