Abstract Abstract: Based on network pharmacology, the mechanism of Danggui Sini Decoction on lumbar disc herniation is explored. We systematically searched the Chinese Medicine System Pharmacology Technology Platform (TCMSP) and the Chinese Medicine Potential Target Database (TCM-PTD) to obtain 45 active ingredients and 132 targets of Danggui Sini Decoction, and compared the obtained targets with the Uniprot database. The abbreviation of the loaded gene name was matched. By searching the Human Gene Database (GeneCards) and Mendelian Disease Gene Database (OMIM) disease target database, a total of 379 target of lumbar intervertebral disc herniation was obtained, after importing the table and removing the duplicate target. ClusterProfiler R programming software was used to draw a Venn diagram to obtain 25 targets for the joint action of drugs and diseases. Cytoscape Version 3.6.1 software was used to construct a network of target proteins of the active ingredient of Danggui Sini Decoction-drug disease. The drug-disease co-action target was imported into the functional protein association network platform which is search Tool for Recurring Instances of Neighbouring Genes, STRING, the protein interaction network was obtained, and Cytoscape Version 3.6.1 was used for the protein interaction network topological analysis and annotation analysis, and the work are performed on the graph to obtain core targets AKT1, PTGS2, MAPK1, STAT3, MAPK8, JUN, CASP3, MAPK14, etc. ClusterProfiler R software was used to perform GO function enrichment analysis and KEGG pathway enrichment analysis on the public action targets of disease drugs, and Cytoscape Version 3.6.1 was used to draw the target-pathway network diagram. Paeoniflorin, beta-sitosterol, kaempferol, (+)-catechin, stigmasterol, formononetin and licochalcone were obtained from the effective active ingredients of Danggui Sini Decoction and the key action targets, such as IL6, AKT1, PTGS2, MAPK1, STAT3, MAPK8, JUN, CASP3, MAPK14, IL10, etc. It is found that Danggui Sini Decoction mainly showed MAP kinase activity and protein serine threonine tyrosine kinase activity, heme binding, phosphatase binding, oxidoreductase activity, FMN binding, endopeptidase activity, hormone binding and other functions, involving HIF-1, FoxO, NF-κB, MAPK, signal pathways such as p35 treat lumbar disc herniation. This article reveals the mechanism of action of Danggui Sini Decoction in treating lumbar disc herniation from the perspective of multiple components, multiple targets, and multiple pathways, providing basis and new ideas for new drug development and clinical application.
Key words: Danggui Sini Decoction; lumbar disc herniation; network pharmacology; target; molecular docking
（Acta Laser Biology Sinica, 2021, 30(3): 236-249）
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