Abstract：Abstract: Seeds of leguminous plants often exhibit physical dormancy due to hard seededness, yet the impact of their seed coat constituents on germination remains unclear. In this study, we investigated the effects of seed coat extracts from two important leguminous species, Caragana korshinskii Kom, and Cajanus cajan L., on rice (Oryza sativa L.) seed germination. Rice seeds were germinated on substrates containing seed coat extracts of C. korshinskii or C. cajan at mass concentrations of 0.05 g/mL and 0.10 g/mL, respectively, with distilled water (H2O) serving as the control. Germination indices were statistically analyzed, and physiological parameters — including malondialdehyde (MDA) content, superoxide anion (O2–) levels, antioxidant enzyme activities, and related gene expression — were measured in rice embryos after 48 hours of imbibition under different treatments. The results demonstrated that seed coat extracts from both leguminous species inhibited rice seed germination, with C. korshinskii exhibiting significantly stronger suppression than C. cajan. Compared to the H2O control, embryos treated with seed coat extracts showed elevated MDA and O2– levels, with higher accumulation observed in the C. korshinskii treatment. Antioxidant enzyme activities and related gene expression were significantly suppressed in the C. korshinskii group, whereas the C. cajan group displayed mixed responses, suggesting incomplete inhibition of the antioxidant system. In conclusion, differences in the composition of seed coat constituents between C. korshinskii and C. cajan may lead to varying degrees of suppression on the antioxidant capacity of rice embryos during germination, resulting in differential oxidative damage and ultimately affecting seed germination. The underlying regulatory mechanisms require further investigation. This study provides a scientific basis for elucidating the physiological mechanisms by which seed coats influence seed germination.
Key word: leguminous plants; seed coat extract; seed germination; antioxidation
（Acta Laser Biology Sinica, 2025, 34(2): 157-164）
引用本文:
许欣悦，齐曼瑶，薛 华，邓志军. 柠条和木豆种皮内含物抑制种子萌发的抗氧化生理研究[J]. 激光生物学报, 2025, 34(2): 157-164.
XU Xinyue, QI Manyao, XUE Hua, DENG Zhijun. A Study on Antioxidant Regulation of the Inhibition of Seed Germination by the Seed Coat Contents of Caragana korshinskii and Cajanus cajan. journal1, 2025, 34(2): 157-164.
