Abstract：Abstract: This study aims to explore the biocontrol effects of tobacco root exudates and their components on Myzus persicae. The composition of tobacco root exudates was identified and analyzed using gas chromatography-mass spectrometry (GC-MS) technology, and their contact toxicity against Myzus persicae was evaluated. The results showed that a total of 63 compounds were detected in tobacco root exudates at different growth stages and under different extraction solvents. These compounds mainly included hydrocarbons (21 types), lipids (10 types), phenols (6 types), and organic acids (5 types). As the growth period of tobacco increased, both the variety and relative content of compounds in the root exudates significantly increased. On the 20th day after transplanting, 30 and 14 compounds were identified using dichloromethane (DCM) and ethyl acetate (EAC) as extraction solvents, respectively. By the 40th day, the number of compounds increased to 54 and 33, respectively. DCM extracted the highest number of compounds, especially hydrocarbons, while EAC extracted more organic acids and lipids. Tobacco root exhibited significant toxicity and inhibitory effects against Myzus persicae, with insecticidal effects increasing as the mass concentration of exudates and the duration of treatment increased. When the mass concentration of tobacco root exudates reached 5 g/mL, the mortality rate of tobacco aphid treated for 24 h was more than 88.53%. Among the tobacco root exudates extracted by the two reagents, the toxic effect of DCM extraction was superior to EAC. Additionally, diisobutyl phthalate (DIBP), a component of the tobacco root exudates, also demonstrated good contact toxicity against Myzus persicae, with a corrected mortality rate of 88.04% after 48 h at a mass concentration of 1 g/mL. The corrected mortality rate increased with higher mass concentrations. This study showed that tobacco root exudates and their components had significant contact effects on Myzus persicae, and the results provided scientific basis for the development of plant protection agents of tobacco root exudates and the application of green control strategies such as tobacco planting.
Key words: tobacco; Myzus persicae; root exudates; contact toxicity; green prevention and control
（Acta Laser Biology Sinica, 2025, 34(2): 132-139）
引用本文:
魏维科，张晋千，杨馨澜，杨丽华*，刘介雄，邓新红，杨 远. 烟草根系分泌物的鉴定及其对烟蚜的抑制作用[J]. 激光生物学报, 2025, 34(2): 132-139.
WEI Weike, ZHANG Jinqian, YANG Xinlan, YANG Lihua*, LIU Jiexiong, DENG Xinhong, YANG Yuan. Identification of Tobacco Root Exudates and Their Inhibitory Effect on Myzus persicae#br#. journal1, 2025, 34(2): 132-139.
