Abstract：To investigate the diversity and structure of bacterial community in the contents of spoiled eggs. Samples were collected from egg protein liquates (J) and smelly eggs (JC), and the 16S rDNA (V4 V5) region amplicons were sequenced by Miseq. The results showed that 42 550 and 38 477 highquality sequences were obtained from J and JC, respectively, which were assigned to 19 OTUs, covering 2 phyla, 14 families, 19 genus and 23 species. The J samples had slightly lower bacterial richness, whereas higher community diversity than JC. The dominant taxa were Proteobacteria Enterobacteriaceae Citrobacter， Serratia and Moraxellaceae Acinetobacter，Pseudomonadaceae Pseudomonas， Firmicutes Bacillaceae Bacillus and Carnobacteriaceae Carnobacterium；The relative abundance of Bacillus was the highest (40.10%) in J samples, whereas unclassified at the species level. In JC samples, the relative abundance of Citrobacter was the highest (81.23%), mainly Citrobacter gillenii, and the abundance of EscherichiaShigella and Enterococcus was very low. There are 14 unclassified or uncultured, and the same species belonged to different OTU in 23 species at the species level. The bacterial population of spoiled eggs is complex, and its diversity of community and abundance have an effect on the spoilage of eggs, which can provide a reference for the further study of the main spoilage bacteria of eggs and their control.
