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Abstract (Affiliated Hospital of Xuzhou Medical University, Xuzhou 221000, China)
Abstract: Acute pancreatitis（AP） is one of the most common gastrointestinal diseases requiring urgent hospitalization, and its incidence is on the rise. This study aims to evaluate whether empagliflozin (EMPA) can alleviate AP in rats by inhibiting apoptosis. Eighteen SPF-grade SD male rats were randomly divided into three groups using a random number table method: control group (CON group), AP group, and AP+EMPA group, with six rats in each group. After successful establishment of the rat models, blood and pancreatic samples were collected. The serum markers tested included amylase (AMY), glucose (GLU), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) levels. Western blot analysis was used to detect the expression levels of Caspase-3 and Bcl2 in the pancreatic tissue, while immunohistochemistry and immunofluorescence were used to assess Caspase-3 expression in the pancreatic tissue. Pancreatic tissues were paraffin-embedded for hematoxylin-eosin (HE) staining and pathological scoring. One-way ANOVA was employed for statistical analysis of the data. The study found that the AP group had elevated serum AMY, GLU, inflammatory cytokine levels, and pathological scores compared to the CON group. In the pancreatic tissue, the apoptosis-related molecule Caspase-3 was elevated, while the expression level of Bcl2 was reduced compared to the CON group (P<0.05). However, EMPA treatment could reverse these changes. In conclusion, EMPA effectively ameliorates the pathological changes in the pancreatic tissue in a rat model of AP, and this protective effect may be related to the inhibition of apoptosis. This article aims to explore the potential therapeutic effects and molecular mechanisms of EMPA in AP, providing new insights for the effective prevention and treatment of this disease.
Key words: acute pancreatitis; apoptosis; Caspase-3; empagliflozin; rat model
（Acta Laser Biology Sinica, 2024, 33(6): 550-556）
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. [J]. journal1, 2024, 33(6): 1-6. |
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