Abstract: Bacterial diseases of fish pose a serious threat to the development of fish breeding industry, and the abuse of antibiotics and the emergence of drug resistance of pathogenic bacteria have a serious impact on the yield of fish breeding, the quality of aquatic products and the breeding environment. In order to promote the development of healthy fish breeding industry, it is urgent to innovate the green prevention and control technology of fish diseases. As a natural and non-residual bacterial killer, phage has the characteristics of strong specificity and high lysis efficiency. Using phage to treat bacterial diseases of fish will be an important technical approach. This paper reviews the important resource mining of phages, the mechanism of action in the prevention and control of bacterial diseases in fish and its application prospects, and puts forward measures to speed up the research of phage treatment technology in the field of healthy aquaculture of fish, which is of great significance for healthy aquaculture of fish.

Key words: fish farming; phage therapy; bacterial diseases; bacterial killer; green prevention and control technology

（Acta Laser Biology Sinica, 2023, 32(6): 517-524）