Abstract：In the present study, highpurity linolenic acid products were obtained by twopass chromatography of linseed oil with ureasilica gel and cuprous chloridesilica gel. The results showed that linseed oil contained only oleic acid, linoleic acid and linolenic acid after ureasilica chromatography. A linolenic acid product with a purity higher than 90% was obtained after cuprous chloridesilica chromatography. Methyl linolenic acid was used as the standard for the external standard quantitative method. The total linolenic acid of the sample showed good linearity in the range of 0.045〖KG-*4〗~0.677 mg/mL. The regression equation was Y=782 59x-686 8(R2=0.999 9). This research will provide relevant theoretical basis and new ideas for the purification and industrial production of linolenic acid.
