Abstract：Abstract: Pheophorbide being a oxygenase gene (PaO) encoded a key enzyme in the process of chlorophyll degradation. In this study, in order to understand the characteristics of the TaPaOs gene family in wheat (Triticum aestivum L.) and the role it plays in the aging process, the published genome-wide information of wheat and other species was used to analyze the genetic features of the TaPaOs family, preliminarily identified their functions by real-time fluorescence quantitative (qRT-PCR) technology. The results showed that a total of 18 TaPaOs family members were found in wheat, distributed on 15 chromosomes. The encoded proteins were hydrophilic, most of which were unstable proteins, and their subcellular localization was predicted in chloroplasts. The promoter regions of the 18 family members contained a large number of response elements, among which the light response element (G-Box) and ABA response element (ABRE) were the most. The expression patterns of the TaPaOs gene family in different tissues were analyzed and found that leaves were the most, followed by stems and lowest roots. Most of the family members upregulated expression during ABA-induced aging, dark induced aging, and natural aging, especially TaPaO2, TaPaO5, and TaPaO6 would be used as the focus of wheat aging research, which laid the foundation for further study of the TaPaoOs gene family function in wheat.
Key words: wheat; TaPaOs gene family; chlorophyll; expression analysis; aging
（Acta Laser Biology Sinica, 2023, 32(1): 043-054）
引用本文:
闫荣岳，郝辉芳，晋秀娟，郭 峰，许成杰，汤小莎，王曙光，范 华，孙黛珍. 小麦脱镁叶绿酸a加氧酶基因家族鉴定及其响应衰老特征分析[J]. 激光生物学报, 2023, 32(1): 43-54.
YAN Rongyue, HAO Huifang, JIN Xiujuan, GUO Feng, XU Chengjie, TANG Xiaosha, WANG Shuguang, FAN Hua, SUN Daizhen. Familial Identification of Wheat Pheophorbide a Oxygenase Gene and Its Characteristic Analysis in Response to Aging. journal1, 2023, 32(1): 43-54.
