Abstract：Functional constipation is a common intestinal disease in recent years. Increasing evidence shows that some biologically active substances has a positive effect on the treatment of constipation. Spirulina is rich in polysaccharide which have a number of physiological functions. This study was to investigate the therapeutic effects of polysaccharide from Spirulina platensis (PSP) on constipation mice induced by diphenoxylate. Different does of PSP were used to feed in the mice. Examinations on the mice included body weight, first defecation time and number, intestinal enzyme activity, bacterial composition and related functional gene expression. The results showed that the number of mouse defecations treatments increased significantly (P<0.05) within six hours, and the time for the first blackout was shortened in the low, medium and high dose groups of PSP. At the same time, PSP had no significant effect on the growth of mouse body weight. After PSP treatment, the xylanase and protease activities in the intestine of constipation mice restored to levels in normal group (P<0.05). In addition, PSP treatment resulted in the changes in the composition of the main microflora in the intestinal tract of constipation mice, followed by upregulating the abundance of some beneficial functional genes and sequentially leading to the medication effect on constipation mice. Notably PSP treatment upregulates the abundance level of KO0845 (glucokinase) and maintains the body’s blood sugar balance. PSP feeding can significantly improve intestinal microecology, anesis constipation in mice and provide an effective diet for chronic constipation.
